Role of pH in solubility and conformational changes of Pacific whiting muscle proteins

Supawan Thawornchinsombut, Jae W. Park

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study was conducted to better understand biochemical changes offish muscle proteins as affected by novel surimi process, acid- or alkali-aided solubilization. At 10 mM NaCl, between pH 5 and 10, the solubility of Pacific whiting muscle proteins was low but increased dramatically as the pH was shifted to either acidic or alkaline pH. At 600 mM NaCl, the isoelectric point was shifted to the acidic direction by about 2 pH units, resulting in aggregation of proteins at low pH, but improving the solubility of MHC (myosin heavy chain) between pH 6 and 10. ANS surface hydrophobidty (ANS-S o showed much greater values than PRODAN surface hydrophobidty (PRODAN-S o) for samples treated at pH 2-4 perhaps due to an enhancement of the electrostatic interactions between the ANS probe and proteins. At very high pH, according to hydrophobicity results, proteins were partially refolded when the ionic strength increased. Under acidic conditions, SDS-PAGE demonstrated the degradation of MHC at 10 mM NaCl. The formation of MHC polymers was observed under alkaline treatment with a concomitant decrease of SH content.

Original languageEnglish
Pages (from-to)135-154
Number of pages20
JournalJournal of Food Biochemistry
Volume28
Issue number2
Publication statusPublished - 2004 May 1
Externally publishedYes

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Merluccius productus
Myosin Heavy Chains
Muscle Proteins
muscle protein
Solubility
solubility
myosin heavy chains
Proteins
Alkalies
Hydrophobicity
Coulomb interactions
Ionic strength
Polymers
Agglomeration
Degradation
Acids
surimi
electrostatic interactions
proteins
Isoelectric Point

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Role of pH in solubility and conformational changes of Pacific whiting muscle proteins. / Thawornchinsombut, Supawan; Park, Jae W.

In: Journal of Food Biochemistry, Vol. 28, No. 2, 01.05.2004, p. 135-154.

Research output: Contribution to journalArticle

Thawornchinsombut, Supawan ; Park, Jae W. / Role of pH in solubility and conformational changes of Pacific whiting muscle proteins. In: Journal of Food Biochemistry. 2004 ; Vol. 28, No. 2. pp. 135-154.
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