Role of phospholipase C-γ1 in insulin-like growth factor I-induced muscle differentiation of H9c2 cardiac myoblasts

Feng Hong, Keun ai Moon, Sam Soo Kim, Young Seol Kim, Young Kil Choi, Yun Soo Bae, Pann Ghill Suh, Sung Ho Ryu, Eui Ju Choi, Joohun Ha, Sung Soo Kim

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20 Citations (Scopus)

Abstract

Insulin-like growth factor-I (IGF-I) regulates muscle differentiation through phosphatidylinositol 3-kinase (PI 3-kinase). Also it was recently reported that PI 3-kinase is involved in the activation of phospholipase C-γ1 (PLC-γ1). We investigated whether PLC-γ1 therefore plays a role in IGF-I-induced muscle differentiation using H9c2 rat cardiac myoblasts as a model. IGF-I was able to activate PLC-γ1 via both PI 3-kinase-dependent and tyrosine phosphorylation-dependent mechanisms in this model. However, PI 3-kinase appeared to play a more important role than tyrosine phosphorylation in IGF-I activation of PLC-γ1. In addition, PLC-γ1 activation was independent of Akt/protein kinase B (Akt/PKB). Importantly, PLC-γ1 was involved in IGF-I-induced muscle differentiation in parallel with Akt/PKB. Taken together, these results suggest that IGF-I regulation of muscle differentiation is dependent on the activation of PLC-γ1 and Akt/PKB, both of which are downstream mediators of PI 3-kinase.

Original languageEnglish
Pages (from-to)816-822
Number of pages7
JournalBiochemical and biophysical research communications
Volume282
Issue number3
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hong, F., Moon, K. A., Kim, S. S., Kim, Y. S., Choi, Y. K., Bae, Y. S., Suh, P. G., Ryu, S. H., Choi, E. J., Ha, J., & Kim, S. S. (2001). Role of phospholipase C-γ1 in insulin-like growth factor I-induced muscle differentiation of H9c2 cardiac myoblasts. Biochemical and biophysical research communications, 282(3), 816-822. https://doi.org/10.1006/bbrc.2001.4644