Role of Rac GTPase in the nuclear signaling by EGF

Byung Chul Kim, Jae Hong Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The role of Rac in epidermal growth factor (EGF)-induced c-fos serum response element (SRE) activation was examined in Rat-2 fibroblast cells. By reporter gene analysis following transient or stable transfections, with pEXV-RacN17 encoding a dominant-negative mutant of Rac, EGF-induced activation of c-fos SRE-luciferase gene was shown to be selectively inhibited, suggesting that Rac activity is necessary for the full activation of SRE by EGF. Our further study to analyze the downstream mediator of Rac in EGF-signaling cascade demonstrated that there is a functional link between Rac and phospholipase A2 (PLA2) activation and further that PLA2 mediates, at least partly, the Rac signaling to SRF. Together, our results point to a critical role of Rac and Rac-activated PLA2 in the EGF-signaling cascade to c-fos SRE. We propose that 'Rac-PLA2' cascade is one of the major signaling pathways by which EGF stimulates c-fos SRE.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalFEBS Letters
Volume407
Issue number1
DOIs
Publication statusPublished - 1997 Apr 21

Keywords

  • C-fos
  • Epidermal growth factor
  • Phospholipase A
  • Rac GTPase
  • Serum response element
  • Signal transduction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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