Role of secreted type I collagen derived from stromal cells in two breast cancer cell lines

Sung Hoon Kim, Hye Yoon Lee, Seung Pil Jung, Sangmin Kim, Jeong Eon Lee, Seok Jin Nam, Jeoung Won Bae

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Collagen is one of numerous components of the cellular microenvironment. To date, the association between the microenvironment and tumorigenesis of malignant breast cancer remains elusive. Therefore, the aim of the present study was to investigate the potential role of a secretory protein of stromal cells, type I collagen, in the development of the aggressive characteristics of breast cancer cells. MDA-MB231 and MCF7 breast cancer cell lines were maintained in cultured media of normal human dermal fibroblasts (HDFs) and type I collagen-containing media. The morphological changes, adhesion capacity and matrix metalloproteinase (MMP)-1, -2 and -9 mRNA levels were evaluated. The results revealed that cell sprouting and adhesion capacity were enhanced in the MCF7 and MDA-MB231 breast cancer cells in HDF-conditioned culture media as well as in response to type I collagen treatment. The expression of MMP-9 mRNA was high in breast cancer cells cultured with the media of normal HDFs, compared with that of the control media. These data indicate that type I collagen, which is secreted by stromal fibroblasts, may augment the aggressive characteristics of breast cancer cells through the induction of MMP-9 mRNA.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalOncology Letters
Volume8
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Stromal Cells
Collagen Type I
Breast Neoplasms
Cell Line
Fibroblasts
Matrix Metalloproteinase 9
Messenger RNA
Skin
Cellular Microenvironment
Matrix Metalloproteinase 1
Matrix Metalloproteinase 2
Conditioned Culture Medium
Cell Adhesion
Cultured Cells
Carcinogenesis
Collagen
Proteins

Keywords

  • Breast cancer
  • Collagen type I
  • Fibroblast
  • Matrix metalloproteinases
  • Neoplasm

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Role of secreted type I collagen derived from stromal cells in two breast cancer cell lines. / Kim, Sung Hoon; Lee, Hye Yoon; Jung, Seung Pil; Kim, Sangmin; Lee, Jeong Eon; Nam, Seok Jin; Bae, Jeoung Won.

In: Oncology Letters, Vol. 8, No. 2, 01.01.2014, p. 507-512.

Research output: Contribution to journalArticle

Kim, Sung Hoon ; Lee, Hye Yoon ; Jung, Seung Pil ; Kim, Sangmin ; Lee, Jeong Eon ; Nam, Seok Jin ; Bae, Jeoung Won. / Role of secreted type I collagen derived from stromal cells in two breast cancer cell lines. In: Oncology Letters. 2014 ; Vol. 8, No. 2. pp. 507-512.
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