Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages

Seung Hyun Yang, Dan Heo, Jinsung Park, Sung Soo Na, Jin Suck Suh, Seungjoo Haam, Sahng Wook Park, Yong Min Huh, Jaemoon Yang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.

Original languageEnglish
Article number505702
JournalNanotechnology
Volume23
Issue number50
DOIs
Publication statusPublished - 2012 Dec 21

Fingerprint

Macrophages
Cytotoxicity
Surface charge
Nanoparticles
Manganese
Ferrite
Polysorbates
Surface-Active Agents
Surface active agents
manganese ferrite
Water
Cell Communication
Hydroxyl Radical
Solubility
Amines
Stiffness
Membranes

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages. / Yang, Seung Hyun; Heo, Dan; Park, Jinsung; Na, Sung Soo; Suh, Jin Suck; Haam, Seungjoo; Wook Park, Sahng; Huh, Yong Min; Yang, Jaemoon.

In: Nanotechnology, Vol. 23, No. 50, 505702, 21.12.2012.

Research output: Contribution to journalArticle

Yang, SH, Heo, D, Park, J, Na, SS, Suh, JS, Haam, S, Wook Park, S, Huh, YM & Yang, J 2012, 'Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages', Nanotechnology, vol. 23, no. 50, 505702. https://doi.org/10.1088/0957-4484/23/50/505702
Yang, Seung Hyun ; Heo, Dan ; Park, Jinsung ; Na, Sung Soo ; Suh, Jin Suck ; Haam, Seungjoo ; Wook Park, Sahng ; Huh, Yong Min ; Yang, Jaemoon. / Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages. In: Nanotechnology. 2012 ; Vol. 23, No. 50.
@article{c45c69b49e744fcf92e8ecae2e2535ae,
title = "Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages",
abstract = "Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.",
author = "Yang, {Seung Hyun} and Dan Heo and Jinsung Park and Na, {Sung Soo} and Suh, {Jin Suck} and Seungjoo Haam and {Wook Park}, Sahng and Huh, {Yong Min} and Jaemoon Yang",
year = "2012",
month = "12",
day = "21",
doi = "10.1088/0957-4484/23/50/505702",
language = "English",
volume = "23",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "50",

}

TY - JOUR

T1 - Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages

AU - Yang, Seung Hyun

AU - Heo, Dan

AU - Park, Jinsung

AU - Na, Sung Soo

AU - Suh, Jin Suck

AU - Haam, Seungjoo

AU - Wook Park, Sahng

AU - Huh, Yong Min

AU - Yang, Jaemoon

PY - 2012/12/21

Y1 - 2012/12/21

N2 - Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.

AB - Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.

UR - http://www.scopus.com/inward/record.url?scp=84870423036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870423036&partnerID=8YFLogxK

U2 - 10.1088/0957-4484/23/50/505702

DO - 10.1088/0957-4484/23/50/505702

M3 - Article

C2 - 23164999

AN - SCOPUS:84870423036

VL - 23

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 50

M1 - 505702

ER -