Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis

Sun Jae Kwon, Soon Il Kwon, Min Seok Bae, Eun Ju Cho, Ohkmae Kim

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Methionine residues of proteins are a major target for oxidation by reactive oxygen species (ROS), which are generated in response to a variety of stress conditions. Methionine sulfoxide (MetO) reductases are present in most organisms and play protective roles in the cellular response to oxidative stress, reducing oxidized MetO back to Met. Previously, an Arabidopsis MetO reductase, MsrB3, was identified as a cold-responsive protein. Here we report that MsrB3 functions in the process of cold acclimation, thus contributing to cold tolerance. In contrast to normal, wild-type plants, msrb3 mutant plants lost the ability to become tolerant to freezing temperatures following cold pre-treatment. Furthermore, when exposed to low temperature, msrb3 plants exhibited a larger increase in MetO and H 2O 2 content and electrolyte leakage compared with wild-type and MsrB3 transgenic plants. It is also shown that MsrB3 is localized at the endoplasmic reticulum (ER). We propose that MsrB3 plays an important role in cold tolerance by eliminating MetO and ROS that accumulate at the ER during cold acclimation.

Original languageEnglish
Pages (from-to)1713-1723
Number of pages11
JournalPlant and Cell Physiology
Volume48
Issue number12
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

Acclimatization
Arabidopsis
methionine
acclimation
cold tolerance
endoplasmic reticulum
reactive oxygen species
Endoplasmic Reticulum
Reactive Oxygen Species
Methionine Sulfoxide Reductases
freezing point
electrolytes
transgenic plants
oxidative stress
proteins
pretreatment
Genetically Modified Plants
oxidation
Methionine
mutants

Keywords

  • Arabidopsis
  • Cold acclimation
  • Cold stress
  • Met sulfoxide reductase
  • Oxidative stress
  • Reactive oxygen species

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis. / Kwon, Sun Jae; Kwon, Soon Il; Bae, Min Seok; Cho, Eun Ju; Kim, Ohkmae.

In: Plant and Cell Physiology, Vol. 48, No. 12, 01.12.2007, p. 1713-1723.

Research output: Contribution to journalArticle

Kwon, Sun Jae ; Kwon, Soon Il ; Bae, Min Seok ; Cho, Eun Ju ; Kim, Ohkmae. / Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 12. pp. 1713-1723.
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