Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics

Gi Young Lee, Deok Gyun You, Hye Ra Lee, Sun Wook Hwang, Changjoon Lee, Young Do Yoo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) modulator 1 (Romo1) is a nuclear-encoded mitochondrial inner membrane protein known to regulate mitochondrial ROS production and to act as an essential redox sensor in mitochondrial dynamics. Although its physiological roles have been studied for a decade, the biophysical mechanisms that explain these activities of Romo1 are unclear. In this study, we report that Romo1 is a unique mitochondrial ion channel that differs from currently identified eukaryotic ion channels. Romo1 is a highly conserved protein with structural features of class II viroporins, which are virusencoded nonselective cation channels. Indeed, Romo1 forms a nonselective cation channel with its amphipathic helical transmembrane domain necessary for pore-forming activity. Notably, channel activity was specifically inhibited by Fe2+ ions, an essential transition metal ion in ROS metabolism. Using structural bioinformatics, we designed an experimental data- guided structural model of Romo1 with a rational hexameric structure. We propose that Romo1 establishes a new category of viroporin-like nonselective cation channel in eukaryotes.

Original languageEnglish
Pages (from-to)2059-2071
Number of pages13
JournalJournal of Cell Biology
Volume217
Issue number6
DOIs
Publication statusPublished - 2018 Jun 1

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Cations
Reactive Oxygen Species
Ion Channels
Ions
Mitochondrial Dynamics
Structural Models
Computational Biology
Eukaryota
Oxidation-Reduction
Membrane Proteins
Metals
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics. / Lee, Gi Young; You, Deok Gyun; Lee, Hye Ra; Hwang, Sun Wook; Lee, Changjoon; Yoo, Young Do.

In: Journal of Cell Biology, Vol. 217, No. 6, 01.06.2018, p. 2059-2071.

Research output: Contribution to journalArticle

Lee, Gi Young ; You, Deok Gyun ; Lee, Hye Ra ; Hwang, Sun Wook ; Lee, Changjoon ; Yoo, Young Do. / Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics. In: Journal of Cell Biology. 2018 ; Vol. 217, No. 6. pp. 2059-2071.
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AU - Yoo, Young Do

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