Romo1 is a negative-feedback regulator of Myc

Seung Baek Lee, Jung Jin Kim, Jin Sil Chung, Myeong Sok Lee, Kee Ho Lee, Byung Soo Kim, William P. Tansey, Young Do Yoo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Degradation of Myc protein is mediated by E3 ubiquitin ligases, including SCFFbw7 and SCFSkp2, but much remains unknown about the mechanism of S-phase kinase-associated protein (Skp2)-mediated Myc degradation. In the present study, we show that upregulated Myc protein, which triggers the G1-S phase progression in response to growth-stimulatory signals, induces reactive oxygen species modulator 1 (Romo1) expression. Romo1 subsequently triggers Skp2-mediated ubiquitylation and degradation of Myc by a mechanism not previously reported in normal lung fibroblasts. We also show that reactive oxygen species (ROS) derived from steady-state Romo1 expression are necessary for cell cycle entry of quiescent cells. From this study, we suggest that the generation of ROS mediated by pre-existing Romo1 protein is required for Myc induction. Meanwhile, Romo1 expression induced by Myc during G1 phase stimulates Skp2-mediated Myc degradation in a negative-feedback mechanism.

Original languageEnglish
Pages (from-to)1911-1924
Number of pages14
JournalJournal of Cell Science
Volume124
Issue number11
DOIs
Publication statusPublished - 2011 Jun 1

Fingerprint

Reactive Oxygen Species
G1 Phase
S-Phase Kinase-Associated Proteins
Ubiquitin-Protein Ligases
Ubiquitination
S Phase
Proteolysis
Cell Cycle
Proteins
Fibroblasts
Lung
Growth

Keywords

  • Myc
  • Romo1
  • ROS
  • Skp2

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lee, S. B., Kim, J. J., Chung, J. S., Lee, M. S., Lee, K. H., Kim, B. S., ... Yoo, Y. D. (2011). Romo1 is a negative-feedback regulator of Myc. Journal of Cell Science, 124(11), 1911-1924. https://doi.org/10.1242/jcs.079996

Romo1 is a negative-feedback regulator of Myc. / Lee, Seung Baek; Kim, Jung Jin; Chung, Jin Sil; Lee, Myeong Sok; Lee, Kee Ho; Kim, Byung Soo; Tansey, William P.; Yoo, Young Do.

In: Journal of Cell Science, Vol. 124, No. 11, 01.06.2011, p. 1911-1924.

Research output: Contribution to journalArticle

Lee, SB, Kim, JJ, Chung, JS, Lee, MS, Lee, KH, Kim, BS, Tansey, WP & Yoo, YD 2011, 'Romo1 is a negative-feedback regulator of Myc', Journal of Cell Science, vol. 124, no. 11, pp. 1911-1924. https://doi.org/10.1242/jcs.079996
Lee SB, Kim JJ, Chung JS, Lee MS, Lee KH, Kim BS et al. Romo1 is a negative-feedback regulator of Myc. Journal of Cell Science. 2011 Jun 1;124(11):1911-1924. https://doi.org/10.1242/jcs.079996
Lee, Seung Baek ; Kim, Jung Jin ; Chung, Jin Sil ; Lee, Myeong Sok ; Lee, Kee Ho ; Kim, Byung Soo ; Tansey, William P. ; Yoo, Young Do. / Romo1 is a negative-feedback regulator of Myc. In: Journal of Cell Science. 2011 ; Vol. 124, No. 11. pp. 1911-1924.
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