Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome

Ki Moon Seong, Je Hyun Baek, Myeong Hee Yu, Joon Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The 26S proteasome, composed of the 20S core and 19S regulatory complexes, is important for the turnover of polyubiquitinated proteins. Each subunit of the complex plays a special role in proteolytic function, including substrate recruitment, deubiquitination, and structural contribution. To assess the function of some non-essential subunits in the 26S proteasome, we isolated the 26S proteasome from deletion strains of RPN13 and RPN14 using TAP affinity purification. The stability of Gcn4p and the accumulation of ubiquitinated Gcn4p were significantly increased, but the affinity in the recognition of proteasome was decreased. In addition, the subcomplexes of the isolated 26S proteasomes from deletion mutants were less stable than that of the wild type. Taken together, our findings indicate that Rpn13p and Rpn14p are involved in the efficient recognition of 26S proteasome for the proteolysis of ubiquitinated Gcn4p.

Original languageEnglish
Pages (from-to)2567-2573
Number of pages7
JournalFEBS Letters
Volume581
Issue number13
DOIs
Publication statusPublished - 2007 May 29

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Proteolysis
Proteasome Endopeptidase Complex
Purification
ATP dependent 26S protease
Substrates
Proteins

Keywords

  • 19S regulatory particle
  • 26S proteasome
  • Rpn13p
  • Rpn14p
  • Ubiquitinated Gcn4p

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. / Seong, Ki Moon; Baek, Je Hyun; Yu, Myeong Hee; Kim, Joon.

In: FEBS Letters, Vol. 581, No. 13, 29.05.2007, p. 2567-2573.

Research output: Contribution to journalArticle

Seong, Ki Moon ; Baek, Je Hyun ; Yu, Myeong Hee ; Kim, Joon. / Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. In: FEBS Letters. 2007 ; Vol. 581, No. 13. pp. 2567-2573.
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AU - Baek, Je Hyun

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AU - Kim, Joon

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