RPTPμ tyrosine phosphatase promotes adipogenic differentiation via modulation of p120 catenin phosphorylation

Won Kon Kim, Hyeyun Jung, Eun Young Kim, Do Hyung Kim, Yee Sook Cho, Byoung Chul Park, Sung Goo Park, Yong Ko, Kwang Hee Bae, Sang Chul Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Adipocyte differentiation can be regulated by the combined activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). In particular, PTPs act as key regulators in differentiation-associated signaling pathways. We recently found that receptor-type PTPμ (RPTPμ) expression is markedly increased during the adipogenic differentiation of 3T3-L1 preadipocytes and mesenchymal stem cells. Here, we investigate the functional roles of RPTPμ and the mechanism of its involvement in the regulation of signal transduction during adipogenesis of 3T3-L1 cells. Depletion of endogenous RPTPμ by RNA interference significantly inhibited adipogenic differentiation, whereas RPTPμ overexpression led to an increase in adipogenic differentiation. Ectopic expression of p120 catenin suppressed adipocyte differentiation, and the decrease in adipogenesis by p120 catenin was recovered by introducing RPTPμ. Moreover, RPTPμ induced a decrease in the cytoplasmic p120 catenin expression by reducing its tyrosine phosphorylation level, consequently leading to enhanced translocation of Glut-4 to the plasma membrane. On the basis of these results, we propose that RPTPμ acts as a positive regulator of adipogenesis by modulating the cytoplasmic p120 catenin level. Our data conclusively demonstrate that differentiation into adipocytes is controlled by RPTPμ, supporting the utility of RPTPμ and p120 catenin as novel target proteins for the treatment of obesity.

Original languageEnglish
Pages (from-to)4883-4891
Number of pages9
JournalMolecular Biology of the Cell
Volume22
Issue number24
DOIs
Publication statusPublished - 2011 Dec 15

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Phosphoric Monoester Hydrolases
Adipogenesis
Phosphorylation
Protein Tyrosine Phosphatases
Adipocytes
3T3-L1 Cells
RNA Interference
Mesenchymal Stromal Cells
Protein-Tyrosine Kinases
Tyrosine
Signal Transduction
Obesity
Cell Membrane
tyrosine receptor
delta catenin
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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RPTPμ tyrosine phosphatase promotes adipogenic differentiation via modulation of p120 catenin phosphorylation. / Kim, Won Kon; Jung, Hyeyun; Kim, Eun Young; Kim, Do Hyung; Cho, Yee Sook; Park, Byoung Chul; Park, Sung Goo; Ko, Yong; Bae, Kwang Hee; Lee, Sang Chul.

In: Molecular Biology of the Cell, Vol. 22, No. 24, 15.12.2011, p. 4883-4891.

Research output: Contribution to journalArticle

Kim, WK, Jung, H, Kim, EY, Kim, DH, Cho, YS, Park, BC, Park, SG, Ko, Y, Bae, KH & Lee, SC 2011, 'RPTPμ tyrosine phosphatase promotes adipogenic differentiation via modulation of p120 catenin phosphorylation', Molecular Biology of the Cell, vol. 22, no. 24, pp. 4883-4891. https://doi.org/10.1091/mbc.E11-03-0175
Kim, Won Kon ; Jung, Hyeyun ; Kim, Eun Young ; Kim, Do Hyung ; Cho, Yee Sook ; Park, Byoung Chul ; Park, Sung Goo ; Ko, Yong ; Bae, Kwang Hee ; Lee, Sang Chul. / RPTPμ tyrosine phosphatase promotes adipogenic differentiation via modulation of p120 catenin phosphorylation. In: Molecular Biology of the Cell. 2011 ; Vol. 22, No. 24. pp. 4883-4891.
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