RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes

Keh Chien Lee, Yun Hee Jang, Soon Kap Kim, Hyo Young Park, May Phyo Thu, Jeong Hwan Lee, Jeong Kook Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Key message: The RNA recognition motif of Arabidopsis splicing factor SF1 affects the alternative splicing ofFLOWERING LOCUS Mpre-mRNA and a heat shock transcription factorHsfA2pre-mRNA. Abstract: Splicing factor 1 (SF1) plays a crucial role in 3′ splice site recognition by binding directly to the intron branch point. Although plant SF1 proteins possess an RNA recognition motif (RRM) domain that is absent in its fungal and metazoan counterparts, the role of the RRM domain in SF1 function has not been characterized. Here, we show that the RRM domain differentially affects the full function of the Arabidopsis thaliana AtSF1 protein under different experimental conditions. For example, the deletion of RRM domain influences AtSF1-mediated control of flowering time, but not the abscisic acid sensitivity response during seed germination. The alternative splicing of FLOWERING LOCUS M (FLM) pre-mRNA is involved in flowering time control. We found that the RRM domain of AtSF1 protein alters the production of alternatively spliced FLM-β transcripts. We also found that the RRM domain affects the alternative splicing of a heat shock transcription factor HsfA2 pre-mRNA, thereby mediating the heat stress response. Taken together, our results suggest the importance of RRM domain for AtSF1-mediated alternative splicing of a subset of genes involved in the regulation of flowering and adaptation to heat stress.

Original languageEnglish
Pages (from-to)1083-1095
Number of pages13
JournalPlant Cell Reports
Volume36
Issue number7
DOIs
Publication statusPublished - 2017 Jul 1

Fingerprint

Arabidopsis
RNA
alternative splicing
genes
heat stress
flowering
proteins
abscisic acid
introns
seed germination
Arabidopsis thaliana
transcription factors
transcription (genetics)

Keywords

  • Alternative splicing
  • AtSF1
  • FLM
  • Flowering time
  • Heat stress
  • RRM domain

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes. / Lee, Keh Chien; Jang, Yun Hee; Kim, Soon Kap; Park, Hyo Young; Thu, May Phyo; Lee, Jeong Hwan; Kim, Jeong Kook.

In: Plant Cell Reports, Vol. 36, No. 7, 01.07.2017, p. 1083-1095.

Research output: Contribution to journalArticle

Lee, Keh Chien ; Jang, Yun Hee ; Kim, Soon Kap ; Park, Hyo Young ; Thu, May Phyo ; Lee, Jeong Hwan ; Kim, Jeong Kook. / RRM domain of Arabidopsis splicing factor SF1 is important for pre-mRNA splicing of a specific set of genes. In: Plant Cell Reports. 2017 ; Vol. 36, No. 7. pp. 1083-1095.
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