Runella limosa sp. nov., isolated from activated sludge

Seung Hyun Ryu, Thi Thu Hien Nguyen, Woojun Park, Chang Jin Kim, Che Ok Jeon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A Gram-negative bacterium, designated strain EMB111 T, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Cells were long and rod-shaped. The isolate was strictly aerobic and non-motile. The strain grew optimally at 25-30°C and pH 7.5-8.0. The predominant fatty acids of strain EMB111 T were iso-C 15:0, C 16:1ω5c, iso-C 17:0 3-OH, iso-C 15:0 3-OH, C 16:0 3-OH, C 16:0 and summed feature 3 (C 16:1ω7c and/ or iso-C 15:0 2-OH). The strain contained a large amount of phosphatidylglycerol and small amounts of two unknown phospholipids (PL1, PL2) as the polar lipids. The major isoprenoid quinone was menaquinone-7. The G+C content of the genomic DNA was 42.7 mol%. Phylogenetic analysis showed that strain EMB111 T formed a phyletic cluster with members of the genus Runella within the family Flexibacteraceae and was most closely related to Runella slithytormis ATCC 29530 T with a 16S rRNA gene sequence similarity of 94.8%. On the basis of chemotaxonomic data and molecular properties, strain EMB111 T represents a novel species within the genus Runella, for which the name Runelly limosa sp. nov. is proposed. The type strain is EMB111 T (=KCTC 12615 T=DSM 17973 T).

Original languageEnglish
Pages (from-to)2757-2760
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

Runella
activated sludge
isoprenoid
Sewage
phospholipid
genomics
fatty acid
lipid
phosphorus
phylogenetics
DNA
Cytophagaceae
bacterium
gene
new species
Phosphatidylglycerols
Terpenes
Base Composition
Gram-Negative Bacteria
rRNA Genes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Runella limosa sp. nov., isolated from activated sludge. / Ryu, Seung Hyun; Nguyen, Thi Thu Hien; Park, Woojun; Kim, Chang Jin; Jeon, Che Ok.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 56, No. 12, 01.12.2006, p. 2757-2760.

Research output: Contribution to journalArticle

Ryu, Seung Hyun ; Nguyen, Thi Thu Hien ; Park, Woojun ; Kim, Chang Jin ; Jeon, Che Ok. / Runella limosa sp. nov., isolated from activated sludge. In: International Journal of Systematic and Evolutionary Microbiology. 2006 ; Vol. 56, No. 12. pp. 2757-2760.
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