Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT

Sung Ho Lee, Hyung Min Jeong, Jin Myung Choi, Young Chang Cho, Tae Sung Kim, Kwang Youl Lee, Bok Yun Kang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Interleukin (IL)-4 plays a key role in T helper 2 (Th2) cell differentiation favoring humoral immune response. Regulation of IL-4 gene expression, therefore, is critically important for Th2 dependent responses and Th2 dominant disorders. In T cells, IL-4 gene expression is regulated positively or negatively by a combination of several transcription factors. Recently, enhanced IL-4 production was reported in Runx3 knockout mice; this implies negative regulation of IL-4 by Runx3. Runx proteins are transcription factors that have a Runt domain and have essential functions in development. In this study, the molecular mechanism that downregulates IL-4 expression was investigated. Runx3 inhibited IL-4 production in EL-4 T cells stimulated with PMA/ionomycin. Runx3-mediated IL-4 inhibition was NFAT-dependent, and Runx3 was physically associated with NFAT. Therefore, our results suggest that the interaction between NFAT and Runx3 is a mechanism that causes the negative regulation of IL-4, along with previously reported repression by T-bet.

Original languageEnglish
Pages (from-to)214-217
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume381
Issue number2
DOIs
Publication statusPublished - 2009 Apr 3

Fingerprint

T-cells
Interleukin-4
T-Lymphocytes
Gene expression
Core Binding Factor alpha Subunits
Transcription Factors
Gene Expression
Th2 Cells
Ionomycin
Humoral Immunity
Knockout Mice
Cell Differentiation
Down-Regulation

Keywords

  • IL-4
  • NFAT
  • Runx3

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT. / Lee, Sung Ho; Jeong, Hyung Min; Choi, Jin Myung; Cho, Young Chang; Kim, Tae Sung; Lee, Kwang Youl; Kang, Bok Yun.

In: Biochemical and Biophysical Research Communications, Vol. 381, No. 2, 03.04.2009, p. 214-217.

Research output: Contribution to journalArticle

Lee, Sung Ho ; Jeong, Hyung Min ; Choi, Jin Myung ; Cho, Young Chang ; Kim, Tae Sung ; Lee, Kwang Youl ; Kang, Bok Yun. / Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 381, No. 2. pp. 214-217.
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