RUNX3 inhibits the metastasis and angiogenesis of colorectal cancer

Bo Ram Kim, Myoung Hee Kang, Jung Lim Kim, Yoo Jin Na, Seong Hye Park, Sun Il Lee, Sanghee Kang, Sung Yup Joung, Suk Young Lee, Dae Hee Lee, Byung Wook Min, Sang Cheul Oh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recent studies have determined that inactivation of runt-related transcription factor 3 (RUNX3) expression is highly associated with lymph node metastasis and poor prognosis in various types of cancer. However, the mechanism of RUNX3-mediated suppression of tumor metastasis remains unclear. Herein, we aimed to clarify the effect of RUNX3 on metastasis and angiogenesis in colorectal cancer (CRC). Firstly, we found that the reduction in expression of RUNX3 in CRC tissues when compared with tumor adjacent normal colon tissues, as indicated by reduced RUNX3 staining, was significantly correlated with tumor-node-metastasis (TNM) stage. Secondly, we demonstrated that RUNX3 overexpression inhibited CRC cell migration and invasion resulting from the upregulation of matrix metalloproteinase-2 (MMP-2) and MMP-9 expression. In contrast, the knockdown of RUNX3 reduced the inhibition of migration and invasion of CRC cells. Finally, we found that restoration of RUNX3 decreased vascular endothelial growth factor (VEGF) secretion and suppressed endothelial cell growth and tube formation in CRC cells. All in all, our findings may provide insight into the development of RUNX3 for CRC metastasis diagnostics and therapeutics.

Original languageEnglish
Pages (from-to)2601-2608
Number of pages8
JournalOncology Reports
Volume36
Issue number5
DOIs
Publication statusPublished - 2016 Nov 1

Fingerprint

Colorectal Neoplasms
Neoplasm Metastasis
Neoplasms
Transcription Factor 3
Matrix Metalloproteinase 2
Matrix Metalloproteinases
Vascular Endothelial Growth Factor A
Cell Movement
Colon
Up-Regulation
Endothelial Cells
Lymph Nodes
Staining and Labeling
Growth
Therapeutics

Keywords

  • Angiogenesis
  • Colorectal cancer
  • Migration
  • Runt-related transcription factor 3
  • VEGF

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

RUNX3 inhibits the metastasis and angiogenesis of colorectal cancer. / Kim, Bo Ram; Kang, Myoung Hee; Kim, Jung Lim; Na, Yoo Jin; Park, Seong Hye; Lee, Sun Il; Kang, Sanghee; Joung, Sung Yup; Lee, Suk Young; Lee, Dae Hee; Min, Byung Wook; Oh, Sang Cheul.

In: Oncology Reports, Vol. 36, No. 5, 01.11.2016, p. 2601-2608.

Research output: Contribution to journalArticle

Kim, BR, Kang, MH, Kim, JL, Na, YJ, Park, SH, Lee, SI, Kang, S, Joung, SY, Lee, SY, Lee, DH, Min, BW & Oh, SC 2016, 'RUNX3 inhibits the metastasis and angiogenesis of colorectal cancer', Oncology Reports, vol. 36, no. 5, pp. 2601-2608. https://doi.org/10.3892/or.2016.5086
Kim, Bo Ram ; Kang, Myoung Hee ; Kim, Jung Lim ; Na, Yoo Jin ; Park, Seong Hye ; Lee, Sun Il ; Kang, Sanghee ; Joung, Sung Yup ; Lee, Suk Young ; Lee, Dae Hee ; Min, Byung Wook ; Oh, Sang Cheul. / RUNX3 inhibits the metastasis and angiogenesis of colorectal cancer. In: Oncology Reports. 2016 ; Vol. 36, No. 5. pp. 2601-2608.
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