[Ru(phen)2DPPZ]2+ is in contact with DNA bases when it forms a luminescent complex with single-stranded oligonucleotides

Seok Joon Moon, Jong Moon Kim, Ji Youn Choi, Seog K. Kim, Je Seung Lee, Ho Gyeom Jang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The luminescence intensity of the Δ- and Λ-enantiomer of [Ru(phen)2DPPZ]2+ ([Ru(phenanthroline)2 dipyrido[3,2-a:2′,3′-c]phenazine]2+) complex enhanced upon binding to double stranded DNA, which has been known as "light switch effect". The enhancement of the luminescence required the intercalation of the large ligand between DNA base pairs. In this study, we report the enhancement in the luminescence intensity when the metal complexes bind to single stranded oligonucleotides, indicating that the "light switch effect" does not require intercalation of the large DPPZ ligand. Oligonucleotides may provide a hydrophobic cavity for the [Ru(phen) 2DPPZ]2+ complex to prevent the quenching by the water molecule. In the cavity, the metal complex is in contact with DNA bases as is evidenced by the observation that the excited energy of the DNA bases transfer to the bound metal complex. However, the contact of the metal complex with DNA bases is different from the stacking of DPPZ in the intercalation pocket. In addition to the normal two luminescence lifetimes, a short lifetime in the range of 1-2 ns was found for both the Δ- and Λ-enantiomer of [Ru(phen)2DPPZ]2+ when complexed with single stranded oligonucleotides, which may be assigned to the metal complex that is outside of the cavity, interacting with phosphate groups of DNA.

Original languageEnglish
Pages (from-to)994-1000
Number of pages7
JournalJournal of Inorganic Biochemistry
Volume99
Issue number5
DOIs
Publication statusPublished - 2005 May 1

Fingerprint

Oligonucleotides
Coordination Complexes
Luminescence
DNA
Intercalation
Enantiomers
Switches
Ligands
Light
Phenanthrolines
Base Pairing
Quenching
Phosphates
Molecules
Water

Keywords

  • [Ru(phen)DPPZ]
  • Contact energy transfer
  • DNA
  • Light switch

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

[Ru(phen)2DPPZ]2+ is in contact with DNA bases when it forms a luminescent complex with single-stranded oligonucleotides. / Moon, Seok Joon; Kim, Jong Moon; Choi, Ji Youn; Kim, Seog K.; Lee, Je Seung; Jang, Ho Gyeom.

In: Journal of Inorganic Biochemistry, Vol. 99, No. 5, 01.05.2005, p. 994-1000.

Research output: Contribution to journalArticle

Moon, Seok Joon ; Kim, Jong Moon ; Choi, Ji Youn ; Kim, Seog K. ; Lee, Je Seung ; Jang, Ho Gyeom. / [Ru(phen)2DPPZ]2+ is in contact with DNA bases when it forms a luminescent complex with single-stranded oligonucleotides. In: Journal of Inorganic Biochemistry. 2005 ; Vol. 99, No. 5. pp. 994-1000.
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AB - The luminescence intensity of the Δ- and Λ-enantiomer of [Ru(phen)2DPPZ]2+ ([Ru(phenanthroline)2 dipyrido[3,2-a:2′,3′-c]phenazine]2+) complex enhanced upon binding to double stranded DNA, which has been known as "light switch effect". The enhancement of the luminescence required the intercalation of the large ligand between DNA base pairs. In this study, we report the enhancement in the luminescence intensity when the metal complexes bind to single stranded oligonucleotides, indicating that the "light switch effect" does not require intercalation of the large DPPZ ligand. Oligonucleotides may provide a hydrophobic cavity for the [Ru(phen) 2DPPZ]2+ complex to prevent the quenching by the water molecule. In the cavity, the metal complex is in contact with DNA bases as is evidenced by the observation that the excited energy of the DNA bases transfer to the bound metal complex. However, the contact of the metal complex with DNA bases is different from the stacking of DPPZ in the intercalation pocket. In addition to the normal two luminescence lifetimes, a short lifetime in the range of 1-2 ns was found for both the Δ- and Λ-enantiomer of [Ru(phen)2DPPZ]2+ when complexed with single stranded oligonucleotides, which may be assigned to the metal complex that is outside of the cavity, interacting with phosphate groups of DNA.

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