Rutin increases muscle mitochondrial biogenesis with AMPK activation in high-fat diet-induced obese rats

Sangjin Seo, Mak Soon Lee, Eugene Chang, Yoonjin Shin, Soojung Oh, In-Hwan Kim, Yangha Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Decreased mitochondrial number and dysfunction in skeletal muscle are associated with obesity and the progression of obesity-associated metabolic disorders. The specific aim of the current study was to investigate the effects of rutin on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese rats. Supplementation with rutin reduced body weight and adipose tissue mass, despite equivalent energy intake (p < 0.05). Rutin significantly increased mitochondrial size and mitochondrial DNA (mtDNA) content as well as gene expression related to mitochondrial biogenesis, such as peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1α), nuclear respiratory factor-1 (NRF-1), transcription factor A (Tfam), and nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, sirtulin1 (SIRT1) in skeletal muscle (p < 0.05). Moreover, rutin consumption increased muscle adenosine monophosphate-activated protein kinase (AMPK) activity by 40% (p < 0.05). Taken together, these results suggested at least partial involvement of muscle mitochondria and AMPK activation in the rutin-mediated beneficial effect on obesity.

Original languageEnglish
Pages (from-to)8152-8169
Number of pages18
JournalNutrients
Volume7
Issue number9
DOIs
Publication statusPublished - 2015 Sep 22

Fingerprint

AMP-activated protein kinase
Rutin
High Fat Diet
Organelle Biogenesis
Adenosine Monophosphate
rutin
high fat diet
Protein Kinases
Muscles
muscles
rats
skeletal muscle
Skeletal Muscle
obesity
Obesity
Nuclear Respiratory Factor 1
Muscle Mitochondrion
Mitochondrial Size
Peroxisome Proliferator-Activated Receptors
Mitochondrial Genes

Keywords

  • AMPK activity
  • Mitochondria
  • Obesity
  • Rutin
  • Skeletal muscle

ASJC Scopus subject areas

  • Food Science

Cite this

Rutin increases muscle mitochondrial biogenesis with AMPK activation in high-fat diet-induced obese rats. / Seo, Sangjin; Lee, Mak Soon; Chang, Eugene; Shin, Yoonjin; Oh, Soojung; Kim, In-Hwan; Kim, Yangha.

In: Nutrients, Vol. 7, No. 9, 22.09.2015, p. 8152-8169.

Research output: Contribution to journalArticle

Seo, Sangjin ; Lee, Mak Soon ; Chang, Eugene ; Shin, Yoonjin ; Oh, Soojung ; Kim, In-Hwan ; Kim, Yangha. / Rutin increases muscle mitochondrial biogenesis with AMPK activation in high-fat diet-induced obese rats. In: Nutrients. 2015 ; Vol. 7, No. 9. pp. 8152-8169.
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