Rutin increases muscle mitochondrial biogenesis with AMPK activation in high-fat diet-induced obese rats

Sangjin Seo, Mak Soon Lee, Eugene Chang, Yoonjin Shin, Soojung Oh, In-Hwan Kim, Yangha Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)


Decreased mitochondrial number and dysfunction in skeletal muscle are associated with obesity and the progression of obesity-associated metabolic disorders. The specific aim of the current study was to investigate the effects of rutin on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese rats. Supplementation with rutin reduced body weight and adipose tissue mass, despite equivalent energy intake (p < 0.05). Rutin significantly increased mitochondrial size and mitochondrial DNA (mtDNA) content as well as gene expression related to mitochondrial biogenesis, such as peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1α), nuclear respiratory factor-1 (NRF-1), transcription factor A (Tfam), and nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, sirtulin1 (SIRT1) in skeletal muscle (p < 0.05). Moreover, rutin consumption increased muscle adenosine monophosphate-activated protein kinase (AMPK) activity by 40% (p < 0.05). Taken together, these results suggested at least partial involvement of muscle mitochondria and AMPK activation in the rutin-mediated beneficial effect on obesity.

Original languageEnglish
Pages (from-to)8152-8169
Number of pages18
Issue number9
Publication statusPublished - 2015 Sep 22



  • AMPK activity
  • Mitochondria
  • Obesity
  • Rutin
  • Skeletal muscle

ASJC Scopus subject areas

  • Food Science

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