S-nitrosation ameliorates homocysteine-induced neurotoxicity and calcium responses in primary culture of rat cortical neurons

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Homocyteine (HC)induces neurotoxicity through overstimulation of N- methyl-D-aspartate (NMDA) receptors in cortical neurons. Due to its high reactivity, the sulfhydryl group of HC may react with nitric oxide (NO). In the present study S-nitrosation decreased the homocysteine-evoked LDH releases from rat cortical neuronal cultures. Like HC, S-nitrosohomocysteine increased the intracellular calcium concentration ([Ca2+](i)) via NMDA receptor. However, S-nitrosohomocysteine was much less efficacious than HC for the increase of [Ca2+](i). Elevation of the glycine concentration to 50 μM significantly increased the maximal calcium response of S- nitrosohomocyteine, but not high enough to match that of HC in the presence of the same concentration of glycine. S-nitrosohomocysteine partially decreased the NMDA calcium responses in the presence of I and 50 μM glycine, at least in part via the redox-modulatory site(s) of the NMDA receptor. These data indicate that NO ameliorates the potential, adverse properties of HC via S-nitrosylation in the pathogenesis of hyperhomocysteinemia.

Original languageEnglish
Pages (from-to)99-102
Number of pages4
JournalNeuroscience Letters
Volume265
Issue number2
DOIs
Publication statusPublished - 1999 Apr 16

Keywords

  • Homocysteine
  • N-methyl-D-aspartate receptor
  • Neurotoxicity
  • S-nitrosation
  • S-nitrosohomocysteine
  • [Ca](i), glycine

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'S-nitrosation ameliorates homocysteine-induced neurotoxicity and calcium responses in primary culture of rat cortical neurons'. Together they form a unique fingerprint.

  • Cite this