S-nitrosation ameliorates homocysteine-induced neurotoxicity and calcium responses in primary culture of rat cortical neurons

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Abstract

Homocyteine (HC)induces neurotoxicity through overstimulation of N- methyl-D-aspartate (NMDA) receptors in cortical neurons. Due to its high reactivity, the sulfhydryl group of HC may react with nitric oxide (NO). In the present study S-nitrosation decreased the homocysteine-evoked LDH releases from rat cortical neuronal cultures. Like HC, S-nitrosohomocysteine increased the intracellular calcium concentration ([Ca2+](i)) via NMDA receptor. However, S-nitrosohomocysteine was much less efficacious than HC for the increase of [Ca2+](i). Elevation of the glycine concentration to 50 μM significantly increased the maximal calcium response of S- nitrosohomocyteine, but not high enough to match that of HC in the presence of the same concentration of glycine. S-nitrosohomocysteine partially decreased the NMDA calcium responses in the presence of I and 50 μM glycine, at least in part via the redox-modulatory site(s) of the NMDA receptor. These data indicate that NO ameliorates the potential, adverse properties of HC via S-nitrosylation in the pathogenesis of hyperhomocysteinemia.

Original languageEnglish
Pages (from-to)99-102
Number of pages4
JournalNeuroscience Letters
Volume265
Issue number2
DOIs
Publication statusPublished - 1999 Apr 16
Externally publishedYes

Fingerprint

Nitrosation
Homocysteine
N-Methyl-D-Aspartate Receptors
Glycine
Calcium
Neurons
Nitric Oxide
Hyperhomocysteinemia
N-Methylaspartate
Oxidation-Reduction
S-nitrosohomocysteine

Keywords

  • [Ca](i), glycine
  • Homocysteine
  • N-methyl-D-aspartate receptor
  • Neurotoxicity
  • S-nitrosation
  • S-nitrosohomocysteine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "S-nitrosation ameliorates homocysteine-induced neurotoxicity and calcium responses in primary culture of rat cortical neurons",
abstract = "Homocyteine (HC)induces neurotoxicity through overstimulation of N- methyl-D-aspartate (NMDA) receptors in cortical neurons. Due to its high reactivity, the sulfhydryl group of HC may react with nitric oxide (NO). In the present study S-nitrosation decreased the homocysteine-evoked LDH releases from rat cortical neuronal cultures. Like HC, S-nitrosohomocysteine increased the intracellular calcium concentration ([Ca2+](i)) via NMDA receptor. However, S-nitrosohomocysteine was much less efficacious than HC for the increase of [Ca2+](i). Elevation of the glycine concentration to 50 μM significantly increased the maximal calcium response of S- nitrosohomocyteine, but not high enough to match that of HC in the presence of the same concentration of glycine. S-nitrosohomocysteine partially decreased the NMDA calcium responses in the presence of I and 50 μM glycine, at least in part via the redox-modulatory site(s) of the NMDA receptor. These data indicate that NO ameliorates the potential, adverse properties of HC via S-nitrosylation in the pathogenesis of hyperhomocysteinemia.",
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KW - S-nitrosohomocysteine

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