Abstract
Objective: Diagnosing acute cerebral infarction is crucial in determining prognosis of stroke patients. Although many serologic tests for prompt diagnosis are available, the clinical application of serologic tests is currently limited. We investigated whether S100β, matrix metalloproteinase-9 (MMP-9), D-dimer, and heat shock protein 70 (HSP70) can be used as biomarkers for acute cerebral infarction. Methods: Focal cerebral ischemia was induced using the modified intraluminal filament technique. Mice were randomly assigned to 30-minute occlusion (n=10), 60-minute occlusion (n=10), or sham (n=5) groups. Four hours later, neurological deficits were evaluated and blood samples were obtained. Infarction volumes were calculated and plasma S100β, MMP-9, D-dimer, and HSP70 levels were measured using enzyme-linked immunosorbent assay. Results: The average infarction volume was 12.32±2.31 mm3 and 46.9±7.43 mm3 in the 30-and 60-minute groups, respectively. The mean neurological score in the two ischemic groups was 1.6±0.55 and 3.2±0.70, respectively. S100β, MMP-9, and HSP70 expressions significantly increased after 4 hours of ischemia (p=0.001). Furthermore, S100β and MMP-9 expressions correlated with infarction volumes (p<0.001) and neurological deficits (p<0.001). There was no significant difference in D-dimer expression between groups (p=0.843). The area under the receiver operating characteristic curve (AUC) showed high sensitivity and specificity for MMP-9, HSP70 (AUC=1), and S100β (AUC=0.98). Conclusion: S100β, MMP-9, and HSP70 can complement current diagnostic tools to assess cerebral infarction, suggesting their use as potential biomarkers for acute cerebral infarction.
| Original language | English |
|---|---|
| Pages (from-to) | 548-558 |
| Number of pages | 11 |
| Journal | Journal of Korean Neurosurgical Society |
| Volume | 61 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2018 Sep 1 |
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Keywords
- Acute ischemic stroke
- Biomarkers
- Cerebral infarction volume
ASJC Scopus subject areas
- Surgery
- Neuroscience(all)
- Clinical Neurology
Cite this
S100ß, matrix metalloproteinase-9, D-dimer, and heat shock protein 70 are serologic biomarkers of acute cerebral infarction in a mouse model of transient MCA occlusion. / Choi, Jong Il; Ha, Sung-Kon; Lim, Dong Jun; Kim, Sang-Dae; Kim, Se-Hoon.
In: Journal of Korean Neurosurgical Society, Vol. 61, No. 5, 01.09.2018, p. 548-558.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - S100ß, matrix metalloproteinase-9, D-dimer, and heat shock protein 70 are serologic biomarkers of acute cerebral infarction in a mouse model of transient MCA occlusion
AU - Choi, Jong Il
AU - Ha, Sung-Kon
AU - Lim, Dong Jun
AU - Kim, Sang-Dae
AU - Kim, Se-Hoon
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Objective: Diagnosing acute cerebral infarction is crucial in determining prognosis of stroke patients. Although many serologic tests for prompt diagnosis are available, the clinical application of serologic tests is currently limited. We investigated whether S100β, matrix metalloproteinase-9 (MMP-9), D-dimer, and heat shock protein 70 (HSP70) can be used as biomarkers for acute cerebral infarction. Methods: Focal cerebral ischemia was induced using the modified intraluminal filament technique. Mice were randomly assigned to 30-minute occlusion (n=10), 60-minute occlusion (n=10), or sham (n=5) groups. Four hours later, neurological deficits were evaluated and blood samples were obtained. Infarction volumes were calculated and plasma S100β, MMP-9, D-dimer, and HSP70 levels were measured using enzyme-linked immunosorbent assay. Results: The average infarction volume was 12.32±2.31 mm3 and 46.9±7.43 mm3 in the 30-and 60-minute groups, respectively. The mean neurological score in the two ischemic groups was 1.6±0.55 and 3.2±0.70, respectively. S100β, MMP-9, and HSP70 expressions significantly increased after 4 hours of ischemia (p=0.001). Furthermore, S100β and MMP-9 expressions correlated with infarction volumes (p<0.001) and neurological deficits (p<0.001). There was no significant difference in D-dimer expression between groups (p=0.843). The area under the receiver operating characteristic curve (AUC) showed high sensitivity and specificity for MMP-9, HSP70 (AUC=1), and S100β (AUC=0.98). Conclusion: S100β, MMP-9, and HSP70 can complement current diagnostic tools to assess cerebral infarction, suggesting their use as potential biomarkers for acute cerebral infarction.
AB - Objective: Diagnosing acute cerebral infarction is crucial in determining prognosis of stroke patients. Although many serologic tests for prompt diagnosis are available, the clinical application of serologic tests is currently limited. We investigated whether S100β, matrix metalloproteinase-9 (MMP-9), D-dimer, and heat shock protein 70 (HSP70) can be used as biomarkers for acute cerebral infarction. Methods: Focal cerebral ischemia was induced using the modified intraluminal filament technique. Mice were randomly assigned to 30-minute occlusion (n=10), 60-minute occlusion (n=10), or sham (n=5) groups. Four hours later, neurological deficits were evaluated and blood samples were obtained. Infarction volumes were calculated and plasma S100β, MMP-9, D-dimer, and HSP70 levels were measured using enzyme-linked immunosorbent assay. Results: The average infarction volume was 12.32±2.31 mm3 and 46.9±7.43 mm3 in the 30-and 60-minute groups, respectively. The mean neurological score in the two ischemic groups was 1.6±0.55 and 3.2±0.70, respectively. S100β, MMP-9, and HSP70 expressions significantly increased after 4 hours of ischemia (p=0.001). Furthermore, S100β and MMP-9 expressions correlated with infarction volumes (p<0.001) and neurological deficits (p<0.001). There was no significant difference in D-dimer expression between groups (p=0.843). The area under the receiver operating characteristic curve (AUC) showed high sensitivity and specificity for MMP-9, HSP70 (AUC=1), and S100β (AUC=0.98). Conclusion: S100β, MMP-9, and HSP70 can complement current diagnostic tools to assess cerebral infarction, suggesting their use as potential biomarkers for acute cerebral infarction.
KW - Acute ischemic stroke
KW - Biomarkers
KW - Cerebral infarction volume
UR - http://www.scopus.com/inward/record.url?scp=85056649207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056649207&partnerID=8YFLogxK
U2 - 10.3340/jkns.2017.0200
DO - 10.3340/jkns.2017.0200
M3 - Article
VL - 61
SP - 548
EP - 558
JO - Journal of Korean Neurosurgical Society
JF - Journal of Korean Neurosurgical Society
SN - 2005-3711
IS - 5
ER -