TY - JOUR
T1 - Saccharification of agar using hydrothermal pretreatment and enzymes supplemented with agarolytic β-galactosidase
AU - Lee, Chan Hyoung
AU - Yun, Eun Ju
AU - Kim, Hee Taek
AU - Choi, In Geol
AU - Kim, Kyoung Heon
N1 - Funding Information:
This research was supported by grants of the Fishery Commercialization Technology Development Program of the Ministry of Oceans and Fisheries ( 2012100788 ) and the Advanced Biomass R&D Center of Korea ( 2011-0031353 ) funded through the Korean Government (MSIP). Experiments were performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - The major carbohydrate in red macroalgae is agarose, which is composed of d-galactose and 3,6-anhydro-l-galactose (AHG). So far, agarose saccharification was most efficiently achieved by prehydrolysis using a weak acid and subsequent enzymatic hydrolysis into monomeric sugars. However, this process requires rigorous neutralization after acid prehydrolysis, resulting in the formation of salts. Moreover, residual agarotriose is not further hydrolyzed by currently available β-agarases. In this study, we aimed at eliminating or minimizing the neutralization step by using a low-concentration acid-base buffer such as 20 mM (0.3%, w/v) Tris-HCl for hydrothermal pretreatment of agar at 170 °C for 10 min. In order to hydrolyze agarotriose, an agarolytic β-galactosidase acting on odd-numbered agarooligosaccharides, was introduced into the enzymatic saccharification. Using these chemical and enzymatic processes, the monomeric sugar yields of 44.9% for galactose and 44.8% for AHG on the basis of their theoretical maximum amounts from the initial agar were obtained.
AB - The major carbohydrate in red macroalgae is agarose, which is composed of d-galactose and 3,6-anhydro-l-galactose (AHG). So far, agarose saccharification was most efficiently achieved by prehydrolysis using a weak acid and subsequent enzymatic hydrolysis into monomeric sugars. However, this process requires rigorous neutralization after acid prehydrolysis, resulting in the formation of salts. Moreover, residual agarotriose is not further hydrolyzed by currently available β-agarases. In this study, we aimed at eliminating or minimizing the neutralization step by using a low-concentration acid-base buffer such as 20 mM (0.3%, w/v) Tris-HCl for hydrothermal pretreatment of agar at 170 °C for 10 min. In order to hydrolyze agarotriose, an agarolytic β-galactosidase acting on odd-numbered agarooligosaccharides, was introduced into the enzymatic saccharification. Using these chemical and enzymatic processes, the monomeric sugar yields of 44.9% for galactose and 44.8% for AHG on the basis of their theoretical maximum amounts from the initial agar were obtained.
KW - Agar
KW - Agarolytic β-galactosidase
KW - Hydrothermal pretreatment
KW - Red macroalgae
UR - http://www.scopus.com/inward/record.url?scp=84940577081&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2015.06.017
DO - 10.1016/j.procbio.2015.06.017
M3 - Article
AN - SCOPUS:84940577081
VL - 50
SP - 1629
EP - 1633
JO - Process Biochemistry
JF - Process Biochemistry
SN - 1359-5113
IS - 10
M1 - 10461
ER -