A basidiomycetous fungus, identified as Pholiota adiposa SKU0714 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase and β-glucosidase activities of 26, 32 and 39. U/mL, respectively and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1680, 0.12, 65 and 0.41. U/mL, respectively when grown with rice straw as a carbon source. Among the various plant biomasses tested for saccharification, poplar biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize hydrolysis parameters. A maximum saccharification yield of 83.4% (667. mg/g-substrate), the highest yield from any plant biomass, was obtained with Populus biomass after 24. h of hydrolysis. P. adiposa was proven to be a good choice for the production of reducing sugars from cellulosic biomass.
- Pholiota adiposa
- Populus biomass
- Response surface methodology optimization
ASJC Scopus subject areas
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal