Saccharification of poplar biomass by using lignocellulases from Pholiota adiposa

Sujit Sadashiv Jagtap, Saurabh Sudha Dhiman, Marimuthu Jeya, Yun Chan Kang, Joon Ho Choi, Jung Kul Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A basidiomycetous fungus, identified as Pholiota adiposa SKU0714 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase and β-glucosidase activities of 26, 32 and 39. U/mL, respectively and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1680, 0.12, 65 and 0.41. U/mL, respectively when grown with rice straw as a carbon source. Among the various plant biomasses tested for saccharification, poplar biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize hydrolysis parameters. A maximum saccharification yield of 83.4% (667. mg/g-substrate), the highest yield from any plant biomass, was obtained with Populus biomass after 24. h of hydrolysis. P. adiposa was proven to be a good choice for the production of reducing sugars from cellulosic biomass.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalBioresource Technology
Volume120
DOIs
Publication statusPublished - 2012 Sep 1
Externally publishedYes

Fingerprint

Saccharification
Biomass
biomass
Sugars
hydrolysis
Hydrolysis
sugar
Cellulose 1,4-beta-Cellobiosidase
Glucosidases
Laccase
Cellulase
Straw
Lignin
Fungi
straw
lignin
Carbon
rice
Enzymes
fungus

Keywords

  • Cellulase
  • Pholiota adiposa
  • Populus biomass
  • Response surface methodology optimization
  • Saccharification

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Saccharification of poplar biomass by using lignocellulases from Pholiota adiposa. / Jagtap, Sujit Sadashiv; Dhiman, Saurabh Sudha; Jeya, Marimuthu; Kang, Yun Chan; Choi, Joon Ho; Lee, Jung Kul.

In: Bioresource Technology, Vol. 120, 01.09.2012, p. 264-272.

Research output: Contribution to journalArticle

Jagtap, Sujit Sadashiv ; Dhiman, Saurabh Sudha ; Jeya, Marimuthu ; Kang, Yun Chan ; Choi, Joon Ho ; Lee, Jung Kul. / Saccharification of poplar biomass by using lignocellulases from Pholiota adiposa. In: Bioresource Technology. 2012 ; Vol. 120. pp. 264-272.
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