Saccharification of poplar biomass by using lignocellulases from Pholiota adiposa

Sujit Sadashiv Jagtap, Saurabh Sudha Dhiman, Marimuthu Jeya, Yun Chan Kang, Joon Ho Choi, Jung Kul Lee

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A basidiomycetous fungus, identified as Pholiota adiposa SKU0714 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase and β-glucosidase activities of 26, 32 and 39. U/mL, respectively and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1680, 0.12, 65 and 0.41. U/mL, respectively when grown with rice straw as a carbon source. Among the various plant biomasses tested for saccharification, poplar biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize hydrolysis parameters. A maximum saccharification yield of 83.4% (667. mg/g-substrate), the highest yield from any plant biomass, was obtained with Populus biomass after 24. h of hydrolysis. P. adiposa was proven to be a good choice for the production of reducing sugars from cellulosic biomass.

Original languageEnglish
Pages (from-to)264-272
Number of pages9
JournalBioresource technology
Volume120
DOIs
Publication statusPublished - 2012 Sep
Externally publishedYes

Keywords

  • Cellulase
  • Pholiota adiposa
  • Populus biomass
  • Response surface methodology optimization
  • Saccharification

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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