Safe and Complete Extraction of Astaxanthin from Haematococcus pluvialis by Efficient Mechanical Disruption of Cyst Cell Wall

Muhammad Irshad, Min Eui Hong, Aye Aye Myint, Jaehoon Kim, Sang Jun Sim

Research output: Contribution to journalArticle

Abstract

Haematococcus pluvialis (H. pluvialis) can naturally accumulate high amounts of astaxanthin- A powerful antioxidant. However, complete recovery of astaxanthin from the cysts of H. pluvialis is challenging because of the presence of a robust acetolysis resistant cell wall. Herein, a simple and effective planetary ball-milling pretreatment was developed to rupture the cells to attain an almost complete recovery of astaxanthin from H. pluvialis, using a supercritical CO2 and conventional organic solvent-based extraction. An optimized pretreatment using planetary ball mill under very mild conditions (150 rpm, 60↠min) allowed the recovery of 31.4↠mg/g dry weight of astaxanthin from H. pluvialis, while slightly harsh milling (500 rpm) was found to be detrimental toward astaxanthin. The extracts in acetone exhibited higher antioxidant activity in 1-diphenyl-2-picrylhydrazyl assay than those in dichloromethane. These mild conditions were safe and highly effective for the complete extraction of astaxanthin along with the others extractables. This study opens a new avenue for the efficient recovery of valuable thermolabile ketocarotenoid species from microalgae, which can be applied to the development of economically viable biorefineries.

Original languageEnglish
Article number20190128
JournalInternational Journal of Food Engineering
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Haematococcus pluvialis
astaxanthin
Cell Wall
Cysts
Cells
cell walls
Recovery
Antioxidants
Ball mills
Dichloromethane
Ball milling
pretreatment
Acetone
Organic solvents
Microalgae
biorefining
Assays
Methylene Chloride
methylene chloride
microalgae

Keywords

  • astaxanthin
  • cell wall disruption
  • extraction
  • fatty acids
  • Haematococcus pluvialis

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Engineering (miscellaneous)

Cite this

Safe and Complete Extraction of Astaxanthin from Haematococcus pluvialis by Efficient Mechanical Disruption of Cyst Cell Wall. / Irshad, Muhammad; Hong, Min Eui; Myint, Aye Aye; Kim, Jaehoon; Sim, Sang Jun.

In: International Journal of Food Engineering, 01.01.2019.

Research output: Contribution to journalArticle

@article{73737d89c23741b8a71632c24b94c687,
title = "Safe and Complete Extraction of Astaxanthin from Haematococcus pluvialis by Efficient Mechanical Disruption of Cyst Cell Wall",
abstract = "Haematococcus pluvialis (H. pluvialis) can naturally accumulate high amounts of astaxanthin- A powerful antioxidant. However, complete recovery of astaxanthin from the cysts of H. pluvialis is challenging because of the presence of a robust acetolysis resistant cell wall. Herein, a simple and effective planetary ball-milling pretreatment was developed to rupture the cells to attain an almost complete recovery of astaxanthin from H. pluvialis, using a supercritical CO2 and conventional organic solvent-based extraction. An optimized pretreatment using planetary ball mill under very mild conditions (150 rpm, 60{\^a}† min) allowed the recovery of 31.4{\^a}† mg/g dry weight of astaxanthin from H. pluvialis, while slightly harsh milling (500 rpm) was found to be detrimental toward astaxanthin. The extracts in acetone exhibited higher antioxidant activity in 1-diphenyl-2-picrylhydrazyl assay than those in dichloromethane. These mild conditions were safe and highly effective for the complete extraction of astaxanthin along with the others extractables. This study opens a new avenue for the efficient recovery of valuable thermolabile ketocarotenoid species from microalgae, which can be applied to the development of economically viable biorefineries.",
keywords = "astaxanthin, cell wall disruption, extraction, fatty acids, Haematococcus pluvialis",
author = "Muhammad Irshad and Hong, {Min Eui} and Myint, {Aye Aye} and Jaehoon Kim and Sim, {Sang Jun}",
year = "2019",
month = "1",
day = "1",
doi = "10.1515/ijfe-2019-0128",
language = "English",
journal = "International Journal of Food Engineering",
issn = "1556-3758",
publisher = "Berkeley Electronic Press",

}

TY - JOUR

T1 - Safe and Complete Extraction of Astaxanthin from Haematococcus pluvialis by Efficient Mechanical Disruption of Cyst Cell Wall

AU - Irshad, Muhammad

AU - Hong, Min Eui

AU - Myint, Aye Aye

AU - Kim, Jaehoon

AU - Sim, Sang Jun

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Haematococcus pluvialis (H. pluvialis) can naturally accumulate high amounts of astaxanthin- A powerful antioxidant. However, complete recovery of astaxanthin from the cysts of H. pluvialis is challenging because of the presence of a robust acetolysis resistant cell wall. Herein, a simple and effective planetary ball-milling pretreatment was developed to rupture the cells to attain an almost complete recovery of astaxanthin from H. pluvialis, using a supercritical CO2 and conventional organic solvent-based extraction. An optimized pretreatment using planetary ball mill under very mild conditions (150 rpm, 60↠min) allowed the recovery of 31.4↠mg/g dry weight of astaxanthin from H. pluvialis, while slightly harsh milling (500 rpm) was found to be detrimental toward astaxanthin. The extracts in acetone exhibited higher antioxidant activity in 1-diphenyl-2-picrylhydrazyl assay than those in dichloromethane. These mild conditions were safe and highly effective for the complete extraction of astaxanthin along with the others extractables. This study opens a new avenue for the efficient recovery of valuable thermolabile ketocarotenoid species from microalgae, which can be applied to the development of economically viable biorefineries.

AB - Haematococcus pluvialis (H. pluvialis) can naturally accumulate high amounts of astaxanthin- A powerful antioxidant. However, complete recovery of astaxanthin from the cysts of H. pluvialis is challenging because of the presence of a robust acetolysis resistant cell wall. Herein, a simple and effective planetary ball-milling pretreatment was developed to rupture the cells to attain an almost complete recovery of astaxanthin from H. pluvialis, using a supercritical CO2 and conventional organic solvent-based extraction. An optimized pretreatment using planetary ball mill under very mild conditions (150 rpm, 60↠min) allowed the recovery of 31.4↠mg/g dry weight of astaxanthin from H. pluvialis, while slightly harsh milling (500 rpm) was found to be detrimental toward astaxanthin. The extracts in acetone exhibited higher antioxidant activity in 1-diphenyl-2-picrylhydrazyl assay than those in dichloromethane. These mild conditions were safe and highly effective for the complete extraction of astaxanthin along with the others extractables. This study opens a new avenue for the efficient recovery of valuable thermolabile ketocarotenoid species from microalgae, which can be applied to the development of economically viable biorefineries.

KW - astaxanthin

KW - cell wall disruption

KW - extraction

KW - fatty acids

KW - Haematococcus pluvialis

UR - http://www.scopus.com/inward/record.url?scp=85073162584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073162584&partnerID=8YFLogxK

U2 - 10.1515/ijfe-2019-0128

DO - 10.1515/ijfe-2019-0128

M3 - Article

AN - SCOPUS:85073162584

JO - International Journal of Food Engineering

JF - International Journal of Food Engineering

SN - 1556-3758

M1 - 20190128

ER -