Salting-out effects on the partition of proteins in aqueous two-phase systems

Chan Wha Kim, Cho Kyun Rha

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalJournal of microbiology and biotechnology
Issue number5
Publication statusPublished - 1996 Oct


  • Aqueous two-phase systems
  • Liquid-liquid extraction
  • PEG (polyethylene glycol)
  • Salting-out

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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