Salting-out effects on the partition of proteins in aqueous two-phase systems

Chan Wha Kim, Cho Kyun Rha

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume6
Issue number5
Publication statusPublished - 1996 Oct 1

Fingerprint

Salts
Proteins
Solubility
Polyethylene glycols

Keywords

  • Aqueous two-phase systems
  • Liquid-liquid extraction
  • PEG (polyethylene glycol)
  • Salting-out

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Salting-out effects on the partition of proteins in aqueous two-phase systems. / Kim, Chan Wha; Rha, Cho Kyun.

In: Journal of Microbiology and Biotechnology, Vol. 6, No. 5, 01.10.1996, p. 352-357.

Research output: Contribution to journalArticle

@article{dfbb0f3a098d4c8190a2c8df65994458,
title = "Salting-out effects on the partition of proteins in aqueous two-phase systems",
abstract = "The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.",
keywords = "Aqueous two-phase systems, Liquid-liquid extraction, PEG (polyethylene glycol), Salting-out",
author = "Kim, {Chan Wha} and Rha, {Cho Kyun}",
year = "1996",
month = "10",
day = "1",
language = "English",
volume = "6",
pages = "352--357",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "5",

}

TY - JOUR

T1 - Salting-out effects on the partition of proteins in aqueous two-phase systems

AU - Kim, Chan Wha

AU - Rha, Cho Kyun

PY - 1996/10/1

Y1 - 1996/10/1

N2 - The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.

AB - The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.

KW - Aqueous two-phase systems

KW - Liquid-liquid extraction

KW - PEG (polyethylene glycol)

KW - Salting-out

UR - http://www.scopus.com/inward/record.url?scp=0342300484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342300484&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 352

EP - 357

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 5

ER -