Sample preparation method for plasma membrane proteome analysis

Seung A. Park, Mi Ryung Kim, Pan Kyeom Kim, Hyung D. Cho, Gi Yeon Han, Chan Wha Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.

Original languageEnglish
Pages (from-to)177-180
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume872
Issue number1-2
DOIs
Publication statusPublished - 2008 Sep 1

Fingerprint

Proteome
Cell membranes
Cell Membrane
Ultracentrifugation
Electrophoresis, Gel, Two-Dimensional
Hydrophobicity
Electrophoresis
Hydrophobic and Hydrophilic Interactions
Gels
Proteins

Keywords

  • hMSCs
  • Percoll
  • Plasma membrane
  • Two-dimensional electrophoresis
  • Two-phase extraction
  • Ultracentrifugation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sample preparation method for plasma membrane proteome analysis. / Park, Seung A.; Kim, Mi Ryung; Kim, Pan Kyeom; Cho, Hyung D.; Han, Gi Yeon; Kim, Chan Wha.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 872, No. 1-2, 01.09.2008, p. 177-180.

Research output: Contribution to journalArticle

Park, Seung A. ; Kim, Mi Ryung ; Kim, Pan Kyeom ; Cho, Hyung D. ; Han, Gi Yeon ; Kim, Chan Wha. / Sample preparation method for plasma membrane proteome analysis. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008 ; Vol. 872, No. 1-2. pp. 177-180.
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