Sample preparation method for plasma membrane proteome analysis

Seung Ah Park, Mi Ryung Kim, Pan Kyeom Kim, Hyung Don Cho, Gi Yeon Han, Chan Wha Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.

Original languageEnglish
Pages (from-to)177-180
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume872
Issue number1-2
DOIs
Publication statusPublished - 2008 Sep 1

Keywords

  • Percoll
  • Plasma membrane
  • Two-dimensional electrophoresis
  • Two-phase extraction
  • Ultracentrifugation
  • hMSCs

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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