Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)

S. J. Kim, S. J. Lee, B. D. Kim, K. H. Paek

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalPlant Cell Reports
Volume16
Issue number12
DOIs
Publication statusPublished - 1997 Jan 1

Keywords

  • Cucumber mosaic virus
  • Local lesion assay
  • Satellite-RNA-mediated protection
  • Transgenic hot pepper plants

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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