SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells

Seunghee Yoon, Rony Seger, Eui Ju Choi, Young Sook Yoo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.

Original languageEnglish
Pages (from-to)799-805
Number of pages7
JournalBiochemistry (Moscow)
Volume69
Issue number7
DOIs
Publication statusPublished - 2004 Jul

Keywords

  • B-Raf
  • ERK
  • Neurite outgrowth
  • Nuclear translocation
  • PC12 cells
  • SB203580

ASJC Scopus subject areas

  • Biochemistry

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