Scalable Three-Dimensional Recording Electrodes for Probing Biological Tissues

Jung Min Lee, Dingchang Lin, Guosong Hong, Kyoung Ho Kim, Hong Gyu Park, Charles M. Lieber

Research output: Contribution to journalArticlepeer-review


Electrophysiological recording technologies can provide critical insight into the function of the nervous system and other biological tissues. Standard silicon-based probes have limitations, including single-sided recording sites and intrinsic instabilities due to the probe stiffness. Here, we demonstrate high-performance neural recording using double-sided three-dimensional (3D) electrodes integrated in an ultraflexible bioinspired open mesh structure, allowing electrodes to sample fully the 3D interconnected tissue of the brain. In vivo electrophysiological recording using 3D electrodes shows statistically significant increases in the number of neurons per electrode, average spike amplitudes, and signal to noise ratios in comparison to standard two-dimensional electrodes, while achieving stable detection of single-neuron activity over months. The capability of these 3D electrodes is further shown for chronic recording from retinal ganglion cells in mice. This approach opens new opportunities for a comprehensive 3D interrogation, stimulation, and understanding of the complex circuitry of the brain and other electrogenic tissues in live animals over extended time periods.

Original languageEnglish
Pages (from-to)4552-4559
Number of pages8
JournalNano Letters
Issue number11
Publication statusPublished - 2022 Jun 8


  • double-sided electrodes
  • flexible neural probes
  • injectable probes
  • stable chronic recording
  • ultraflexible mesh electronics

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


Dive into the research topics of 'Scalable Three-Dimensional Recording Electrodes for Probing Biological Tissues'. Together they form a unique fingerprint.

Cite this