Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication

Rak Kyun Seong, Seong wook Seo, Ji Ae Kim, Sarah J. Fletcher, Neil V. Morgan, Mukesh Kumar, Young Ki Choi, Ok Shin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Schlafen (SLFN) proteins have been suggested to play important functions in cell proliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhanced viral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-β signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.

Original languageEnglish
JournalImmunobiology
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Antiviral Agents
DNA Viruses
Virus Diseases
RNA Helicases
Interferon Type I
Human Herpesvirus 3
RNA Viruses
Orthomyxoviridae
Interferons
Cytoplasm
Macrophages
Cell Proliferation
Viruses
Proteins
A549 Cells

Keywords

  • Anti-viral
  • Influenza
  • Interferon
  • SLFN14
  • VZV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

Cite this

Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication. / Seong, Rak Kyun; Seo, Seong wook; Kim, Ji Ae; Fletcher, Sarah J.; Morgan, Neil V.; Kumar, Mukesh; Choi, Young Ki; Shin, Ok.

In: Immunobiology, 2017.

Research output: Contribution to journalArticle

Seong, Rak Kyun ; Seo, Seong wook ; Kim, Ji Ae ; Fletcher, Sarah J. ; Morgan, Neil V. ; Kumar, Mukesh ; Choi, Young Ki ; Shin, Ok. / Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication. In: Immunobiology. 2017.
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AU - Morgan, Neil V.

AU - Kumar, Mukesh

AU - Choi, Young Ki

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AB - Schlafen (SLFN) proteins have been suggested to play important functions in cell proliferation and immune cell development. In this study, we determined the antiviral activities of putative RNA-helicase domain-containing SLFN14. Murine SLFN14 expression was specifically induced by TLR3-mediated pathways and type I interferon (IFN) in RAW264.7 mouse macrophages. To examine the role of SLFN during viral infection, cells were infected with either wild-type PR8 or delNS1/PR8 virus. SLFN14 expression was specifically induced following influenza virus infection. Overexpression of SLFN14 in A549 cells reduced viral replication, whereas knockdown of SLFN14 in RAW264.7 cells enhanced viral titers. Furthermore, SLFN14 promoted the delay in viral NP translocation from cytoplasm to nucleus and enhanced RIG-I-mediated IFN-β signaling. In addition, SLFN14 overexpression promoted antiviral activity against varicella zoster virus (VZV), a DNA virus. In conclusion, our data suggest that SLFN14 is a novel antiviral factor for both DNA and RNA viruses.

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