Screening and characterization of an Agrobacterium tumefaciens mutant strain producing high level of coenzyme Q 10

Tae Su Kim, Ji Hyun Yoo, Sang Yong Kim, Cheol Ho Pan, Vipin C. Kalia, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A mutant of Agrobacterium tumefaciens (S02-13) producing high levels of coenzyme Q 10 (CoQ 10 ), was selected by high-throughput screening after repeated NTG mutagenesis. Mutant S02-13 was resistant to sodium azide and menadione and showed an eight-fold increase in CoQ 10 production, as compared to that of the parent strain. The amount of CoQ 10 produced by this mutant reached 350 mg l -1 in pH-stat fed-batch culture. Mutant S02-13 differed from wild-type in morphology, biochemical properties, and proteome profiles. Scanning electron microscopy results revealed a remarkable change in morphology: the wild-type strain had a rod shape, whereas the mutant S02-13 was coccoid. In contrast to wild-type, the mutant S02-13 strain showed a negative reaction to tryptophan deaminase and N 2 reduction, and a positive reaction to nitrate production in API kit assays. The spots representing proteins that were increased in the proteome expression of the mutant S02-13 strain were identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glucosaminitol dehydrogenase (GlcNOH), and superoxide dismutase (SOD). In particular, the enzyme activities of GAPDH and GlcNOH increased eight-fold and three-fold, respectively, in the mutant, as compared to the wild-type.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalProcess Biochemistry
Volume50
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • 2-Dimensional electrophoresis
  • Coenzyme Q
  • Mutagenesis
  • Production

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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