Screening of new bioactive materials from microbial extracts of soil microorganism (I) antimicrobial activity from 200 samples using microdilution assay

Sang Oun Jung, Joon Kim, Il Moo Chang, Jae Chun Ryu

Research output: Contribution to journalArticle


The microdilution assay recommended by NCCLS (National Committee for Clinical Laboratory Standards) is one of the standardized methods of antibiotic susceptibility test. This method has been widely used clinically to obtain MIC values of antibiotics on pathogenic microorganisms. It is more convinient, rapid and simple to test many samples than other test methods such as agar diffusion assay and broth macrodilution assay. The screening of antimicrobial agents from microbial extracts is too laborious in its process. Therefore, a number of screening methods having more simple procedure have been developed. In our laboratory, we applied microdilution assay for screening the antimicrobial agents. This assay showed dose-response results and was more sensitive than disc diffusion assay in our system. We tested 200 samples of microbial extracts originated from 100 microbial strains and selected several samples as potential candidates. In this report, we show that the microdilution assay is more convenient method in screeing of antibiotic susceptibility than those previously reported.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalArchives of Pharmacal Research
Issue number3
Publication statusPublished - 1998 Jun 1



  • Antibiotics
  • Microdilution assay
  • Secondary Metabolites
  • Soil microorganisms
  • Susceptibility test

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

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