SDF-1α peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment

Muhammad Shafiq, Deling Kong, Soo Hyun Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1α)-derived peptide and heparin tethered poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1α peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1α peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group. SDF-1 and SDF-1/heparin grafts also show more numbers of laminin+ blood vessels, CD90+ stem cells, and alpha smooth muscle actin+ cells than the control group. However, SDF-1 and SDF-1/heparin groups did not significantly differ in terms of blood vessel regeneration and stem cell recruitment. Evaluation of the inflammatory response reveal less numbers of CD68+ macrophages in SDF-1 and SDF-1/heparin groups compared with the control group; whereas three groups show similar numbers of CD206+ macrophages. These results indicate that completely synthetic, cell-free grafts can attract endogenous cells and enhance tissue repair. Bioactive polyesters can be fabricated into different shapes and structures for various tissue engineering applications.

Original languageEnglish
JournalJournal of Biomedical Materials Research - Part A
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Chemokine CXCL12
Polyesters
Peptides
Repair
Tissue
Heparin
Blood vessels
Macrophages
Stem cells
Grafts
Copolymers
Biocompatibility
Tissue engineering
Infiltration
Scaffolds
Muscle
Amino acids
Rats
Assays
Tolonium Chloride

Keywords

  • in situ tissue regeneration
  • Polyester
  • SDF1-α
  • Stem cell recruitment
  • Vascular graft

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

SDF-1α peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment. / Shafiq, Muhammad; Kong, Deling; Kim, Soo Hyun.

In: Journal of Biomedical Materials Research - Part A, 2017.

Research output: Contribution to journalArticle

@article{88ae7c9f371844ed906b2e161e85018a,
title = "SDF-1α peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment",
abstract = "The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1α)-derived peptide and heparin tethered poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1α peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1α peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group. SDF-1 and SDF-1/heparin grafts also show more numbers of laminin+ blood vessels, CD90+ stem cells, and alpha smooth muscle actin+ cells than the control group. However, SDF-1 and SDF-1/heparin groups did not significantly differ in terms of blood vessel regeneration and stem cell recruitment. Evaluation of the inflammatory response reveal less numbers of CD68+ macrophages in SDF-1 and SDF-1/heparin groups compared with the control group; whereas three groups show similar numbers of CD206+ macrophages. These results indicate that completely synthetic, cell-free grafts can attract endogenous cells and enhance tissue repair. Bioactive polyesters can be fabricated into different shapes and structures for various tissue engineering applications.",
keywords = "in situ tissue regeneration, Polyester, SDF1-α, Stem cell recruitment, Vascular graft",
author = "Muhammad Shafiq and Deling Kong and Kim, {Soo Hyun}",
year = "2017",
doi = "10.1002/jbm.a.36130",
language = "English",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",

}

TY - JOUR

T1 - SDF-1α peptide tethered polyester facilitates tissue repair by endogenous cell mobilization and recruitment

AU - Shafiq, Muhammad

AU - Kong, Deling

AU - Kim, Soo Hyun

PY - 2017

Y1 - 2017

N2 - The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1α)-derived peptide and heparin tethered poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1α peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1α peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group. SDF-1 and SDF-1/heparin grafts also show more numbers of laminin+ blood vessels, CD90+ stem cells, and alpha smooth muscle actin+ cells than the control group. However, SDF-1 and SDF-1/heparin groups did not significantly differ in terms of blood vessel regeneration and stem cell recruitment. Evaluation of the inflammatory response reveal less numbers of CD68+ macrophages in SDF-1 and SDF-1/heparin groups compared with the control group; whereas three groups show similar numbers of CD206+ macrophages. These results indicate that completely synthetic, cell-free grafts can attract endogenous cells and enhance tissue repair. Bioactive polyesters can be fabricated into different shapes and structures for various tissue engineering applications.

AB - The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1α)-derived peptide and heparin tethered poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1α peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1α peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group. SDF-1 and SDF-1/heparin grafts also show more numbers of laminin+ blood vessels, CD90+ stem cells, and alpha smooth muscle actin+ cells than the control group. However, SDF-1 and SDF-1/heparin groups did not significantly differ in terms of blood vessel regeneration and stem cell recruitment. Evaluation of the inflammatory response reveal less numbers of CD68+ macrophages in SDF-1 and SDF-1/heparin groups compared with the control group; whereas three groups show similar numbers of CD206+ macrophages. These results indicate that completely synthetic, cell-free grafts can attract endogenous cells and enhance tissue repair. Bioactive polyesters can be fabricated into different shapes and structures for various tissue engineering applications.

KW - in situ tissue regeneration

KW - Polyester

KW - SDF1-α

KW - Stem cell recruitment

KW - Vascular graft

UR - http://www.scopus.com/inward/record.url?scp=85021207086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021207086&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.36130

DO - 10.1002/jbm.a.36130

M3 - Article

C2 - 28571106

AN - SCOPUS:85021207086

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

ER -