Secretin induces neurite outgrowth of PC12 through cAMP-mitogen-activated protein kinase pathway

Hyeon Soo Kim, Sanatombi Yumkham, Sun Hee Kim, Kyungmoo Yea, Chan Shin You, Ho Ryu Sung, Pann Ghill Suh

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The gastrointestinal functions of secretin have been fairly well established. However, its function and mode of action within the nervous system remain largely unclear. To gain insight into this area, we have attempted to determine the effects of secretin on neuronal differentiation. Here, we report that secretin induces the generation of neurite outgrowth in pheochromocytoma PC12 cells. The expressions of Tau and beta-tubulin, neuronal differentiation markers, are increased upon secretin stimulation. In addition, secretin induces sustained mitogen-activated protein kinase (MAPK) activation and also stimulates the cAMP secretion. Moreover, the neurite outgrowth elicited by secretin is suppressed to a marked degree in the presence of either PD98059, a specific MAPK/ERK kinase (MEK) inhibitor, or H89, a specific protein kinase A (PKA) inhibitor. Taken together, these observations demonstrate that secretin induces neurite outgrowth of PC12 cells through cAMP- MAPK pathway, and provide a novel insight into the manner in which secretin participates in neuritogenesis.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalExperimental and Molecular Medicine
Volume38
Issue number1
Publication statusPublished - 2006 Feb 28
Externally publishedYes

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Keywords

  • Mitogen-activated protein kinases
  • Neurites
  • Pheochromocytoma
  • Secretin
  • Tau proteins

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Kim, H. S., Yumkham, S., Kim, S. H., Yea, K., You, C. S., Sung, H. R., & Suh, P. G. (2006). Secretin induces neurite outgrowth of PC12 through cAMP-mitogen-activated protein kinase pathway. Experimental and Molecular Medicine, 38(1), 85-93.