Secretion of adenylate kinase 1 is required for extracellular ATP synthesis in C2C12 myotubes

Hyo Jung Choo, Bong Woo Kim, Oh Bong Kwon, Seok Lee Chang, Jong Soon Choi, Young-Gyu Ko

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Extracellular ATP (exATP) has been known to be a critical ligand regulating skeletal muscle differentiation and contractibility. ExATP synthesis was greatly increased with the high level of adenylate kinase 1 (AK1) and ATP synthase β during C2C12 myogenesis. The exATP synthesis was abolished by the knock-down of AK1 but not by that of ATP synthase β in C2C12 myotubes, suggesting that AK1 is required for exATP synthesis in myotubes. However, membrane-bound AK1β was not involved in exATP synthesis because its expression level was decreased during myogenesis in spite of its localization in the lipid rafts that contain various kinds of receptors and mediate cell signal transduction, cell migration, and differentiation. Interestingly, cytoplasmic AK1 was secreted from C2C12 myotubes but not from C2C12 myoblasts. Taken together all these data, we can conclude that AK1 secretion is required for the exATP generation in myotubes.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalExperimental and Molecular Medicine
Volume40
Issue number2
DOIs
Publication statusPublished - 2008 Apr 30

Fingerprint

Skeletal Muscle Fibers
Adenosine Triphosphate
Muscle Development
Signal transduction
Myoblasts
adenylate kinase 1
Cell Movement
Muscle
Cell Differentiation
Signal Transduction
Skeletal Muscle
Ligands
Membranes
Lipids

Keywords

  • Adenosine triphosphate
  • Adenylate kinase 1
  • ATP synthase
  • Membrane microdomains
  • Muscle development

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Secretion of adenylate kinase 1 is required for extracellular ATP synthesis in C2C12 myotubes. / Choo, Hyo Jung; Kim, Bong Woo; Kwon, Oh Bong; Chang, Seok Lee; Choi, Jong Soon; Ko, Young-Gyu.

In: Experimental and Molecular Medicine, Vol. 40, No. 2, 30.04.2008, p. 220-228.

Research output: Contribution to journalArticle

Choo, Hyo Jung ; Kim, Bong Woo ; Kwon, Oh Bong ; Chang, Seok Lee ; Choi, Jong Soon ; Ko, Young-Gyu. / Secretion of adenylate kinase 1 is required for extracellular ATP synthesis in C2C12 myotubes. In: Experimental and Molecular Medicine. 2008 ; Vol. 40, No. 2. pp. 220-228.
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