Secretion of particles of hepatitis B surface antigen from insect cells using a baculovirus vector.

C. Y. Kang, D. H. Bishop, J. S. Seo, Y. Matsuura, MuHyeon Choe

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa californica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. Spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis B surface antigen. The particles had morphological and antigenic properties identical to those of 22 nm particles isolated from the plasma of chronic active hepatitis patients.

Original languageEnglish
JournalJournal of General Virology
Volume68
Publication statusPublished - 1987 Oct 1
Externally publishedYes

Fingerprint

Nucleopolyhedrovirus
Baculoviridae
Hepatitis B Surface Antigens
Insects
Spodoptera
Chronic Hepatitis
Hepatitis B virus
Genes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Secretion of particles of hepatitis B surface antigen from insect cells using a baculovirus vector. / Kang, C. Y.; Bishop, D. H.; Seo, J. S.; Matsuura, Y.; Choe, MuHyeon.

In: Journal of General Virology, Vol. 68, 01.10.1987.

Research output: Contribution to journalArticle

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