Secretome analysis reveals an Arabidopsis lipase involved in defense against Altemaria brassicicola

Il Seok Oh, Ae Ran Park, Min Seok Bae, Sun Jae Kwon, Young Soon Kim, Ji Eun Lee, Na Young Kang, Sumin Lee, Hyeonsook Cheong, Ohkmae Kim

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

The Arabidopsis thaliana secretome was analyzed by the proteomic approach, which led to the identification of secreted proteins implicated in many aspects of cell biology. We then investigated the change in the Arabidopsis secretome in response to salicylic acid and identified several proteins involved in pathogen response. One of these, a secreted lipase with a GDSL-like motif designated GDSL LIPASE1 (GLIP1), was further characterized for its function in disease resistance, glip1 plants were markedly more susceptible to infection by the necrotrophic fungus Altemaria brassicicola compared with the parental wild-type plants. The recombinant GLIP1 protein possessed lipase and antimicrobial activities that directly disrupt fungal spore integrity. Furthermore, GLIP1 appeared to trigger systemic resistance signaling in plants when challenged with A. brassicicola, because pretreatment of the glip1 mutant with recombinant GLIP1 protein inhibited A. brassicicola-induced cell death in both peripheral and distal leaves. Moreover, glip1 showed altered expression of defense- and ethylene-related genes. GLIP1 transcription was increased by ethephon, the ethylene releaser, but not by salicylic acid or jasmonic acid. These results suggest that GLIP1, in association with ethylene signaling, may be a critical component in plant resistance to A. brassicicola.

Original languageEnglish
Pages (from-to)2832-2847
Number of pages16
JournalPlant Cell
Volume17
Issue number10
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

Lipase
Arabidopsis
Salicylic Acid
salicylic acid
ethylene releasers
ethylene
Proteins
proteins
Cytology
Plant Structures
Disease Resistance
Fungal Spores
fungal spores
Mycoses
biological resistance
ethephon
Pathogens
Cell death
Transcription
jasmonic acid

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Secretome analysis reveals an Arabidopsis lipase involved in defense against Altemaria brassicicola. / Oh, Il Seok; Park, Ae Ran; Bae, Min Seok; Kwon, Sun Jae; Kim, Young Soon; Lee, Ji Eun; Kang, Na Young; Lee, Sumin; Cheong, Hyeonsook; Kim, Ohkmae.

In: Plant Cell, Vol. 17, No. 10, 01.12.2005, p. 2832-2847.

Research output: Contribution to journalArticle

Oh, IS, Park, AR, Bae, MS, Kwon, SJ, Kim, YS, Lee, JE, Kang, NY, Lee, S, Cheong, H & Kim, O 2005, 'Secretome analysis reveals an Arabidopsis lipase involved in defense against Altemaria brassicicola', Plant Cell, vol. 17, no. 10, pp. 2832-2847. https://doi.org/10.1105/tpc.105.034819
Oh, Il Seok ; Park, Ae Ran ; Bae, Min Seok ; Kwon, Sun Jae ; Kim, Young Soon ; Lee, Ji Eun ; Kang, Na Young ; Lee, Sumin ; Cheong, Hyeonsook ; Kim, Ohkmae. / Secretome analysis reveals an Arabidopsis lipase involved in defense against Altemaria brassicicola. In: Plant Cell. 2005 ; Vol. 17, No. 10. pp. 2832-2847.
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