Secretory low molecular weight phospholipase A2 plays important roles in cell elongation and shoot gravitropism in Arabidopsis

Hyoung Yool Lee, Sung Chul Bahn, Yoon Mi Kang, Kyu Hee Lee, Hae Jin Kim, Eun Kyeung Noh, Jiwan P. Palta, Jeong Sheop Shin, Stephen B. Ryu

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

To elucidate the cellular functions of phospholipase A2 in plants, an Arabidopsis cDNA encoding a secretory low molecular weight phospholipase A2 (AtsPLA2β) was isolated. Phenotype analyses of transgenic plants showed that overexpression of AtsPLA 2β promotes cell elongation, resulting in prolonged leaf petioles and inflorescence stems, whereas RNA interference-mediated silencing of AtsPLA2β expression retards cell elongation, resulting in shortened leaf petioles and stems. AtsPLA2β is expressed in the cortical, vascular, and endodermal cells of the actively growing tissues of inflorescence stems and hypocotyls. AtsPLA2β then is secreted into the extracellular spaces, where signaling for cell wall acidification is thought to occur. AtsPLA2β-overexpressing or -silenced transgenic plants showed altered gravitropism in inflorescence stems and hypocotyls. AtsPLA2β expression is induced rapidly by auxin treatment and in the curving regions of inflorescence stems undergoing the gravitropic response. These results suggest that AtsPLA2β regulates the process of cell elongation and plays important roles in shoot gravitropism by mediating auxin-induced cell elongation.

Original languageEnglish
Pages (from-to)1990-2002
Number of pages13
JournalPlant Cell
Volume15
Issue number9
DOIs
Publication statusPublished - 2003 Sep 1

Fingerprint

Gravitropism
gravitropism
Phospholipases
Inflorescence
Arabidopsis
Elongation
cell growth
Molecular Weight
Molecular weight
molecular weight
inflorescences
Indoleacetic Acids
stems
shoots
Hypocotyl
Genetically Modified Plants
petioles
hypocotyls
transgenic plants
auxins

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Lee, H. Y., Bahn, S. C., Kang, Y. M., Lee, K. H., Kim, H. J., Noh, E. K., ... Ryu, S. B. (2003). Secretory low molecular weight phospholipase A2 plays important roles in cell elongation and shoot gravitropism in Arabidopsis. Plant Cell, 15(9), 1990-2002. https://doi.org/10.1105/tpc.014423

Secretory low molecular weight phospholipase A2 plays important roles in cell elongation and shoot gravitropism in Arabidopsis. / Lee, Hyoung Yool; Bahn, Sung Chul; Kang, Yoon Mi; Lee, Kyu Hee; Kim, Hae Jin; Noh, Eun Kyeung; Palta, Jiwan P.; Shin, Jeong Sheop; Ryu, Stephen B.

In: Plant Cell, Vol. 15, No. 9, 01.09.2003, p. 1990-2002.

Research output: Contribution to journalArticle

Lee, Hyoung Yool ; Bahn, Sung Chul ; Kang, Yoon Mi ; Lee, Kyu Hee ; Kim, Hae Jin ; Noh, Eun Kyeung ; Palta, Jiwan P. ; Shin, Jeong Sheop ; Ryu, Stephen B. / Secretory low molecular weight phospholipase A2 plays important roles in cell elongation and shoot gravitropism in Arabidopsis. In: Plant Cell. 2003 ; Vol. 15, No. 9. pp. 1990-2002.
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