Sed1p interacts with Arn3p physically and mediates ferrioxamine B uptake in Saccharomyces cerevisiae

Yong Sung Park, Ho Sang Jeong, Ha Chin Sung, Cheol-Won Yun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two-hybrid analysis can be used to study protein function and metabolic pathways. Using yeast two-hybrid analysis to identify a siderophore uptake pathway in the yeast Saccharomyces cerevisiae, we found that the C-terminal part of the cell-wall protein Sed1p interacts with the N-terminal region of Arn3p. To confirm the physical interaction between the Sed1p C-terminal fragment and the hydrophilic N-terminal fragment of Arn3p, we used an in vitro co-immunoprecipitation assay and a growth test of the strain with bait and SED1 plasmids in quadruple amino acid-depleted medium. The expression of SED1 was upregulated by overexpression of AFT1-1up under the control of the GAL promoter. This occurred despite the lack of an Aft1p-binding consensus region on the upstream region of SED1 or a high concentration of free iron. Although free-iron uptake activity in the Δsed1 strain did not differ from that in the parental strain, ferrioxamine bound-iron uptake activity was reduced in the Δsed1 strain. Moreover, the Δsed1 strain showed low viability at high iron concentrations. Taken together, these results suggest that Sed1p mediates siderophore transport and confers iron resistance in S. cerevisiae.

Original languageEnglish
Pages (from-to)150-155
Number of pages6
JournalCurrent Genetics
Volume47
Issue number3
DOIs
Publication statusPublished - 2005 Mar 1

Fingerprint

Saccharomyces cerevisiae
Iron
Siderophores
Yeasts
Metabolic Networks and Pathways
Immunoprecipitation
Cell Wall
Proteins
Plasmids
ferrioxamine B
Amino Acids
Growth

Keywords

  • ARN3
  • Cell wall protein
  • Ferrioxamine B
  • FOB
  • Iron
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

Cite this

Sed1p interacts with Arn3p physically and mediates ferrioxamine B uptake in Saccharomyces cerevisiae. / Park, Yong Sung; Jeong, Ho Sang; Sung, Ha Chin; Yun, Cheol-Won.

In: Current Genetics, Vol. 47, No. 3, 01.03.2005, p. 150-155.

Research output: Contribution to journalArticle

Park, Yong Sung ; Jeong, Ho Sang ; Sung, Ha Chin ; Yun, Cheol-Won. / Sed1p interacts with Arn3p physically and mediates ferrioxamine B uptake in Saccharomyces cerevisiae. In: Current Genetics. 2005 ; Vol. 47, No. 3. pp. 150-155.
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