Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5′-UTR intron

Mi Jung Kim, Heeja Kim, Jeong Sheop Shin, Chung Han Chung, John B. Ohlrogge, Mi Chung Suh

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The regulation of genes involved in primary lipid metabolism in plants is much less well understood than that in many other pathways in plant biology. In the investigation reported here, we have characterized transcriptional regulatory mechanisms controlling seed-specific FAD2 expression in sesame (Sesamum indicum). FAD2 codes for extra-plastidial FAD2 desaturase, which catalyzes the conversion of oleic acid to linoleic acid. Promoter analysis of the sesame FAD2 gene (SeFAD2) using the β-glucuronidase (GUS) reporter system demonstrated that the -660 to -180 promoter region functions as a negative cis-element in the seed-specific expression of the SeFAD2 gene. Sesame and Arabidopsis FAD2 genes harbor one large intron within their 5′-untranslated region. These introns conferred up to 100-fold enhancement of GUS expression in transgenic Arabidopsis tissues as compared with intron-less controls. Prerequisite cis-elements for the SeFAD2 intron-mediated enhancement of gene expression and the promoter-like activity of SeFAD2 intron were identified. SeFAD2 transcripts were induced by abscisic acid (ABA) in developing sesame seeds, and the -660 to -548 and -179 to -53 regions in the SeFAD2 promoter were implicated in ABA-responsive signaling. Theses observations indicate that an intron-mediated regulatory mechanism is involved in controlling not only the seed-specific expression of the SeFAD2 gene but also the expression of plant FAD2 genes, which are essential for the synthesis of polyunsaturated fatty acids.

Original languageEnglish
Pages (from-to)351-368
Number of pages18
JournalMolecular Genetics and Genomics
Volume276
Issue number4
DOIs
Publication statusPublished - 2006 Oct 1

Fingerprint

Sesamum
5' Untranslated Regions
Oleic Acid
Introns
Seed
Seeds
Genes
Abscisic Acid
Glucuronidase
Arabidopsis
Gene Expression
Plant Genes
Linoleic Acid
Unsaturated Fatty Acids
Lipid Metabolism
Genetic Promoter Regions
Ports and harbors
Gene expression

Keywords

  • Arabidopsis thaliana
  • FAD2
  • Intron-mediated enhancement
  • Microsomal oleic acid desaturase
  • Seed-specific promoter
  • Sesamum indicum

ASJC Scopus subject areas

  • Genetics

Cite this

Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5′-UTR intron. / Kim, Mi Jung; Kim, Heeja; Shin, Jeong Sheop; Chung, Chung Han; Ohlrogge, John B.; Suh, Mi Chung.

In: Molecular Genetics and Genomics, Vol. 276, No. 4, 01.10.2006, p. 351-368.

Research output: Contribution to journalArticle

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abstract = "The regulation of genes involved in primary lipid metabolism in plants is much less well understood than that in many other pathways in plant biology. In the investigation reported here, we have characterized transcriptional regulatory mechanisms controlling seed-specific FAD2 expression in sesame (Sesamum indicum). FAD2 codes for extra-plastidial FAD2 desaturase, which catalyzes the conversion of oleic acid to linoleic acid. Promoter analysis of the sesame FAD2 gene (SeFAD2) using the β-glucuronidase (GUS) reporter system demonstrated that the -660 to -180 promoter region functions as a negative cis-element in the seed-specific expression of the SeFAD2 gene. Sesame and Arabidopsis FAD2 genes harbor one large intron within their 5′-untranslated region. These introns conferred up to 100-fold enhancement of GUS expression in transgenic Arabidopsis tissues as compared with intron-less controls. Prerequisite cis-elements for the SeFAD2 intron-mediated enhancement of gene expression and the promoter-like activity of SeFAD2 intron were identified. SeFAD2 transcripts were induced by abscisic acid (ABA) in developing sesame seeds, and the -660 to -548 and -179 to -53 regions in the SeFAD2 promoter were implicated in ABA-responsive signaling. Theses observations indicate that an intron-mediated regulatory mechanism is involved in controlling not only the seed-specific expression of the SeFAD2 gene but also the expression of plant FAD2 genes, which are essential for the synthesis of polyunsaturated fatty acids.",
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