Seed-specific expression of seven arabidopsis promoters

Hee Jeong Jeong, Jun Young Choi, Hyun Young Shin, Jung Myung Bae, Jeong Sheop Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Seeds contain storage compounds, from various carbohydrates to proteins and lipids, which are synthesized during seed development. For the purposes of many plant researches or commercial applications, developing promoter systems expressing specifically in seeds or in particular constituents or tissues/compartments of seeds are indispensable. To screen genes dominantly or specifically expressed in seed tissues, we analyzed Arabidopsis ATH1 microarray data open to the public. Thirty-two candidate genes were selected and their expressions in seed tissues were confirmed by RT-PCR. Finally, seven genes were selected for promoter analysis. The promoters of seven genes were cloned into pBI101 vector and transformed into Arabidopsis to assay histochemical β-glucuronidase (GUS) activity. We found that Pro-at3g03230 promoter drove GUS expression in a chalazal endosperm, Pro-at4g27530:GUS expressed in both chalazal endosperm and embryo, Pro-at4g31830 accelerated GUS expression both in radicle and procambium, Pro-at5g10120 and Pro-at5g16460 drove GUS expression uniquely in embryo, Pro-at5g53100:GUS expressed only in endosperm, and Pro-at5g54000 promoted GUS expression in both embryo and inner integument. These promoters can be used for expressing any genes in specific seed tissues for practical application.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalGene
Volume553
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Arabidopsis thaliana
  • ATH1 microarray
  • Gene expression
  • Promoters
  • Seed tissue-specific

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

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  • Cite this

    Jeong, H. J., Choi, J. Y., Shin, H. Y., Bae, J. M., & Shin, J. S. (2014). Seed-specific expression of seven arabidopsis promoters. Gene, 553(1), 17-23. https://doi.org/10.1016/j.gene.2014.09.051