Selection and characterization of toxic: Aspergillus spore-specific DNA aptamer using spore-SELEX

Jin Woo Seo, Jee Young Kim, Da Hee Kim, Jeong Joo Oh, Young Jun Kim, Gyu Hyeok Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

As airborne spores of toxic Aspergillus species cause mild symptoms to invasive fungal infections, their indoor concentration should be controlled through real-time management. Aptamer-based biosensors could provide economical and simple solutions for point-of-care. In this study, we isolated aptamers binding to the spores of three representative toxic Aspergillus species (A. fumigatus, A. flavus, and A. niger) for the first time, using cell-SELEX (systematic evolution of ligands through exponential enrichment). Among the aptamer candidates, Asp-3 showed a broad and high binding affinity for the Aspergillus spores. Considering the low binding affinity with proteinase-treated spores, we speculated that the Asp-3 binding sites could be possibly associated with cell surface proteins. The high Asp-3 specificity was confirmed by comparing the binding affinity between the Aspergillus target species and other common indoor fungal species. Moreover, we also established quantitative linear relationships between Asp-3 and the spore concentration of each Aspergillus species. Therefore, the selected Asp-3 aptamer, conjugated with detection sensors, could be an effective biorecognition element for the spores of three toxic Aspergillus species.

Original languageEnglish
Pages (from-to)2608-2615
Number of pages8
JournalRSC Advances
Volume11
Issue number5
DOIs
Publication statusPublished - 2021 Jan 12

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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