Selection of 5-methyltryptophan resistant rice mutants from irradiated calli derived from embryos

D. S. Kim, I. S. Lee, C. S. Jang, D. Y. Hyun, Y. W. Seo, Y. I. Lee

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19 Citations (Scopus)


For increasing the contents of specific free amino acids in rice (Oryza sativa L.) cultivar, Donganbyeo, mutant cell lines resistant to growth inhibition by 5-methyltryptophan (5MT) were selected from embryo cultured callus irradiated with 50 Gy of gamma rays. Two lines, M2-1 and M2-2, were obtained from the regenerated plants by selfing. The segregation ratios of resistance and sensitivity in these lines fitted 9:7 and 3:1 ratios, respectively. Considering the agronomic traits, M2-1 was about the same as the original variety in culm length and a little longer or higher in panicle length and number of tillers. However, the fertility of M 2-1 significantly increased compared to the original variety. M 2-2 had an extremely high culm length and tillering capacity. Four 5MT-resistant homozygous M3lines (MR classes), M3-1-40 and M3-1-116 from M2-1 (MR 1), and M3-2-8 and M3-2-12 from M2-2 (MR II), were obtained in the same manner as with the M2 generation. Protein contents of brown rice were increased about 19% and 32% and the total contents of 9 free essential amino acids were 71% and 34% greater than the original variety in the MR I and MR II groups, respectively. However, for the free essential amino acids of polished rice, there was no difference from the original variety.

Original languageEnglish
Pages (from-to)9-19
Number of pages11
Issue number1
Publication statusPublished - 2004


  • 5-methyltryptophan
  • Embryo culture
  • Free amino acid
  • Gamma rays
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture


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