Selection of 5-methyltryptophan resistant rice mutants from irradiated calli derived from embryos

D. S. Kim, I. S. Lee, C. S. Jang, D. Y. Hyun, Y. W. Seo, Y. I. Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

For increasing the contents of specific free amino acids in rice (Oryza sativa L.) cultivar, Donganbyeo, mutant cell lines resistant to growth inhibition by 5-methyltryptophan (5MT) were selected from embryo cultured callus irradiated with 50 Gy of gamma rays. Two lines, M2-1 and M2-2, were obtained from the regenerated plants by selfing. The segregation ratios of resistance and sensitivity in these lines fitted 9:7 and 3:1 ratios, respectively. Considering the agronomic traits, M2-1 was about the same as the original variety in culm length and a little longer or higher in panicle length and number of tillers. However, the fertility of M 2-1 significantly increased compared to the original variety. M 2-2 had an extremely high culm length and tillering capacity. Four 5MT-resistant homozygous M3lines (MR classes), M3-1-40 and M3-1-116 from M2-1 (MR 1), and M3-2-8 and M3-2-12 from M2-2 (MR II), were obtained in the same manner as with the M2 generation. Protein contents of brown rice were increased about 19% and 32% and the total contents of 9 free essential amino acids were 71% and 34% greater than the original variety in the MR I and MR II groups, respectively. However, for the free essential amino acids of polished rice, there was no difference from the original variety.

Original languageEnglish
Pages (from-to)9-19
Number of pages11
JournalEuphytica
Volume135
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • 5-methyltryptophan
  • Embryo culture
  • Free amino acid
  • Gamma rays
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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