Selection of Arabidopsis genes encoding secreted and plasma membrane proteins

Jae Hwan Goo, Ae Ran Park, Woo Jin Park, Ohkmae Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Secreted and plasma membrane proteins play crucial roles in a variety of physiological and developmental processes of multicellular organisms. Systematic cloning of the genes encoding these proteins is therefore of general interest. An effective method of trapping signal sequences was first described by Tashiro et al. (1993), and a similar yet more efficient method was reported by Klein et al. (1996) and Jacobs et al. (1997). In this study, we carried out the latter yeast-based signal sequence trap to clone genes from Arabidopsis thaliana encoding secreted and plasma membrane proteins. Of 144 sequenced cDNA clones, 18% are identical to previously cloned Arabidopsis thaliana genes, 12% are homologous to genes identified from various organisms, and 46% are novel. All of the isolated genes identical or homologous to previously reported genes are either secreted or plasma membrane proteins, and the remaining novel genes appear to contain functional signal sequences based on computer-aided sequence analysis. The full-length cDNA clones of one homologous gene and another novel gene were isolated and sequenced. The deduced amino acid sequences suggest that the former encodes a secreted protein, and the latter encodes a type 1 membrane protein. These results indicate that the signal sequence trap method is effective and useful for the isolation of plant genes encoding secreted and plasma membrane proteins.

Original languageEnglish
Pages (from-to)415-423
Number of pages9
JournalPlant Molecular Biology
Volume41
Issue number3
DOIs
Publication statusPublished - 1999 Oct 1
Externally publishedYes

Fingerprint

Gene encoding
Cell membranes
Arabidopsis
membrane proteins
blood proteins
Blood Proteins
Membrane Proteins
plasma membrane
Genes
Cell Membrane
Protein Sorting Signals
signal peptide
genes
Clone Cells
clones
Complementary DNA
Arabidopsis thaliana
Physiological Phenomena
traps
Plant Genes

Keywords

  • Arabidopsis thaliana
  • Cloning
  • Plasma membrane proteins
  • Secreted proteins
  • Signal sequence

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Selection of Arabidopsis genes encoding secreted and plasma membrane proteins. / Goo, Jae Hwan; Park, Ae Ran; Park, Woo Jin; Kim, Ohkmae.

In: Plant Molecular Biology, Vol. 41, No. 3, 01.10.1999, p. 415-423.

Research output: Contribution to journalArticle

Goo, Jae Hwan ; Park, Ae Ran ; Park, Woo Jin ; Kim, Ohkmae. / Selection of Arabidopsis genes encoding secreted and plasma membrane proteins. In: Plant Molecular Biology. 1999 ; Vol. 41, No. 3. pp. 415-423.
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