Selection of Drosophila genes encoding secreted and membrane proteins

Jae Hwan Goo, Youngwook Ahn, Ohkmae Kim, Woo Jin Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

With the use of a yeast-based signal sequence trap (YSST) method, we screened a Drosophila cDNA library to isolate genes encoding secreted and membrane proteins. Of the 136 unique cDNA clones sequenced, 11 clones (8.1%) are identical to previously known Drosophila genes, 18 clones (13.2%) are homologous to other genes identified in various organisms, and 91 clones (66.9%) are novel. Most of these genes are secreted or membrane proteins, or appear to contain putative signal sequences at their amino termini. This indicates that YSST is an effective tool for the isolation and analysis of Drosophila genes that play roles in intercellular communication.

Original languageEnglish
Pages (from-to)564-568
Number of pages5
JournalMolecules and Cells
Volume9
Issue number5
Publication statusPublished - 1999 Oct 31
Externally publishedYes

Fingerprint

Drosophila
Membrane Proteins
Protein Sorting Signals
Clone Cells
Genes
Yeasts
Gene Library
Complementary DNA
Proteins

Keywords

  • Drosophila melanogaster
  • Membrane Protein
  • Saccharomyces cerevisiae
  • Screening
  • Secreted Protein
  • Signal Sequence

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Selection of Drosophila genes encoding secreted and membrane proteins. / Goo, Jae Hwan; Ahn, Youngwook; Kim, Ohkmae; Park, Woo Jin.

In: Molecules and Cells, Vol. 9, No. 5, 31.10.1999, p. 564-568.

Research output: Contribution to journalArticle

Goo, JH, Ahn, Y, Kim, O & Park, WJ 1999, 'Selection of Drosophila genes encoding secreted and membrane proteins', Molecules and Cells, vol. 9, no. 5, pp. 564-568.
Goo, Jae Hwan ; Ahn, Youngwook ; Kim, Ohkmae ; Park, Woo Jin. / Selection of Drosophila genes encoding secreted and membrane proteins. In: Molecules and Cells. 1999 ; Vol. 9, No. 5. pp. 564-568.
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