Selection of Drosophila genes encoding secreted and membrane proteins

Jae Hwan Goo, Youngwook Ahn, Ohkmae K. Park, Woo Jin Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

With the use of a yeast-based signal sequence trap (YSST) method, we screened a Drosophila cDNA library to isolate genes encoding secreted and membrane proteins. Of the 136 unique cDNA clones sequenced, 11 clones (8.1%) are identical to previously known Drosophila genes, 18 clones (13.2%) are homologous to other genes identified in various organisms, and 91 clones (66.9%) are novel. Most of these genes are secreted or membrane proteins, or appear to contain putative signal sequences at their amino termini. This indicates that YSST is an effective tool for the isolation and analysis of Drosophila genes that play roles in intercellular communication.

Original languageEnglish
Pages (from-to)564-568
Number of pages5
JournalMolecules and cells
Volume9
Issue number5
Publication statusPublished - 1999 Oct 31
Externally publishedYes

Keywords

  • Drosophila melanogaster
  • Membrane Protein
  • Saccharomyces cerevisiae
  • Screening
  • Secreted Protein
  • Signal Sequence

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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