Selection of embryonic stem cell-derived enhanced green fluorescent protein-positive dopamine neurons using the tyrosine hydroxylase promoter is confounded by reporter gene expression in immature cell populations

Eva Hedlund, Jan Pruszak, Andrew Ferree, Angel Viñuela, Sunghoi Hong, Ole Isacson, Kwang Soo Kim

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Transplantation of mouse embryonic stem (mES) cells can restore function in Parkinson disease models, but can generate teratomas. Purification of dopamine neurons derived from embryonic stem cells by fluorescence-activated cell sorting (FACS) could provide a functional cell population for transplantation while eliminating the risk of teratoma formation. Here we used the tyrosine hydroxylase (TH) promoter to drive enhanced green fluorescent protein (eGFP) expression in mES cells. First, we evaluated 2.5-kilobase (kb) and 9-kb TH promoter fragments and showed that clones generated using the 9-kb fragment produced significantly more eGFP+/TH+ neurons. We selected the 9-kb TH clone with the highest eGFP/TH overlap for further differentiation, FACS, and transplantation experiments. Grafts contained large numbers of eGFP+ dopamine neurons of an appropriate phenotype. However, there were also numerous eGFP+ cells that did not express TH and did not have a neuronal morphology. In addition, we found cells in the grafts representing all three germ layers. Based on these findings, we examined the expression of stem cell markers in our eGFP+ population. We found that a majority of eGFP+ cells were stage-specific embryonic antigen-positive (SSEA-1+) and that the genetically engineered clones contained more SSEA-1+ cells after differentiation than the original D3 mES cells. By negative selection of SSEA-1, we could isolate a neuronal eGFP+ population of high purity. These results illustrate the complexity of using genetic selection to purify mES cell-derived dopamine neurons and provide a comprehensive analysis of cell selection strategies based on tyrosine hydroxylase expression.

Original languageEnglish
Pages (from-to)1126-1135
Number of pages10
JournalStem Cells
Volume25
Issue number5
DOIs
Publication statusPublished - 2007 May 1
Externally publishedYes

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Dopaminergic Neurons
Tyrosine 3-Monooxygenase
Embryonic Stem Cells
Reporter Genes
Gene Expression
CD15 Antigens
Population
Clone Cells
Teratoma
Cell Transplantation
Stage-Specific Embryonic Antigens
Flow Cytometry
Transplants
Germ Layers
enhanced green fluorescent protein
Genetic Selection
Parkinson Disease
Cell Differentiation
Stem Cells
Transplantation

Keywords

  • CD-15
  • Fluorescence-activated cell sorting
  • Genetic engineering
  • Parkinson disease
  • Stage-specific embryonic antigen 1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Selection of embryonic stem cell-derived enhanced green fluorescent protein-positive dopamine neurons using the tyrosine hydroxylase promoter is confounded by reporter gene expression in immature cell populations. / Hedlund, Eva; Pruszak, Jan; Ferree, Andrew; Viñuela, Angel; Hong, Sunghoi; Isacson, Ole; Kim, Kwang Soo.

In: Stem Cells, Vol. 25, No. 5, 01.05.2007, p. 1126-1135.

Research output: Contribution to journalArticle

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