Selection of self-priming molecular replicators

Daechan Park, Andrew D. Ellington, Cheulhee Jung

Research output: Contribution to journalArticle

Abstract

Self-priming amplification of oligonucleotides is possible based on foldback of 3 ends, self-priming, and concatemerization, especially in the presence of phosphorothioate linkages. Such a simple replicative mechanism may have led to the accumulation of specific replicators at or near the origin of life. To determine how early replicators may have competed with one another, we have carried out selections with phosphorothiolated hairpins appended to a short random sequence library (N10). Upon the addition of deoxynucleoside triphosphates and a polymerase, concatemers quickly formed, and those random sequences that templated the insertion of purines, especially during initiation, quickly predominated. Over several serial transfers, particular sequences accumulated, and in isolation these were shown to out-compete less efficient replicators.

Original languageEnglish
Pages (from-to)2169-2176
Number of pages8
JournalNucleic acids research
Volume47
Issue number5
DOIs
Publication statusPublished - 2019 Jan 1

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Purines
Insertional Mutagenesis
Oligonucleotides
Libraries
triphosphoric acid
Origin of Life

ASJC Scopus subject areas

  • Genetics

Cite this

Selection of self-priming molecular replicators. / Park, Daechan; Ellington, Andrew D.; Jung, Cheulhee.

In: Nucleic acids research, Vol. 47, No. 5, 01.01.2019, p. 2169-2176.

Research output: Contribution to journalArticle

Park, Daechan ; Ellington, Andrew D. ; Jung, Cheulhee. / Selection of self-priming molecular replicators. In: Nucleic acids research. 2019 ; Vol. 47, No. 5. pp. 2169-2176.
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