Selective adsorption/desorption of nucleic acids on submicron-sized polymeric particles

Chan Wha Kim, C. Rha

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNA can be removed or separated by selective adsorption/desorption on positively charged submicron-sized polymeric particles (SSPP). The selective adsorption of DNA, in the presence of protein, on positively charged SSPP was accomplished by increasing the concentration of potassium phosphate or sodium phosphate. The adsorption of DNA was not affected by the concentration of potassium phosphate or sodium phosphate up to 1.2M. On the other hand, the adsorption of a protein (bovine serum albumin) was completely impeded by 170mM potassium phosphate. DNA adsorbed on SSPP could be desorbed by increasing the concentration of NaCl or KCl; thus it can be recovered. DNA desorbed from SSPP when the concentration of NaCl or KCl was higher than 0.6M. A complete desorption of DNA was achieved at the concentration of NaCl or KCl above 1.2M.

Original languageEnglish
Pages (from-to)1205-1209
Number of pages5
JournalBiotechnology and Bioengineering
Volume33
Issue number9
Publication statusPublished - 1989 Apr 5
Externally publishedYes

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Nucleic acids
Nucleic Acids
Adsorption
Desorption
DNA
Phosphates
Potassium
Sodium
Proteins
Bovine Serum Albumin
potassium phosphate

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Selective adsorption/desorption of nucleic acids on submicron-sized polymeric particles. / Kim, Chan Wha; Rha, C.

In: Biotechnology and Bioengineering, Vol. 33, No. 9, 05.04.1989, p. 1205-1209.

Research output: Contribution to journalArticle

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