Selective adsorption/desorption of nucleic acids on submicron‐sized polymeric particles

Chan Wha Kim, Cho Kyun Rha

Research output: Contribution to journalLetter

13 Citations (Scopus)

Abstract

DNA can be removed or separated by the selective adsorption/desorption on positively charged submicronsized polymeric particles (SSPP). The selective adsorption of DNA, in the presence of protein, on positively charged SSPP was accomplished by increasing the concentration of potassium phosphate or sodium phosphate. The adsorption of DNA was not affected by the concentration of potassium phosphate or sodium phosphate up to 1.2M. On the other hand, the adsoprtion of a protein (bovine serum albumin) was completely impeded by 170mM potassium phosphate. DNA adsorbed on SSPP could be desorbed by increasing the concentration of NaCl or KCl, thus it can be recovered. DNA desorbed from SSPP when the concentration of NaCl or KC was higher than 0.6M. A complete desorption of DNA was achieved at the concentration of NaCl or KCl above 1.2M.

Original languageEnglish
Pages (from-to)1205-1209
Number of pages5
JournalBiotechnology and Bioengineering
Volume33
Issue number9
DOIs
Publication statusPublished - 1989 Apr 5

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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