Selective and Sensitive Sensing of Flame Retardant Chemicals Through Phage Display Discovered Recognition Peptide

Hyo Eon Jin, Chris Zueger, Woo Jae Chung, Winnie Wong, Byung Yang Lee, Seung Wuk Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report a highly selective and sensitive biosensor for the detection of an environmentally toxic molecule, decabrominated diphenyl ether (DBDE), one of the most common congeners of the polybrominated frame retardants (polybrominated diphenyl ether (PBDE)), using newly discovered DBDE peptide receptors integrated with carbon nanotube field-effect transistors (CNT-FET). The specific DBDE peptide receptor was identified using a high-throughput screening process of phage library display. The resulting binding peptide carries an interesting consensus binding pocket with two Trp-His/Asn-Trp repeats, which binds to the DBDE in a multivalent manner. We integrated the novel DBDE binding peptide onto the CNT-FET using polydiacetylene coating materials linked through cysteine-maleimide click chemistry. The resulting biosensor could detect the desired DBDE selectively with a 1 fM detection limit. Our combined approaches of selective receptor discovery, material nanocoating through click chemistry, and integration onto a sensitive CNT-FET electronic sensor for desired target chemicals will pave the way toward the rapid development of portable and easy-to-use biosensors for desired chemicals to protect our health and environment.

Original languageEnglish
Pages (from-to)7697-7703
Number of pages7
JournalNano Letters
Volume15
Issue number11
DOIs
Publication statusPublished - 2015 Nov 11

Fingerprint

Flame Retardants
flame retardants
Bacteriophages
Flame retardants
Peptides
peptides
Ethers
ethers
Display devices
Carbon nanotube field effect transistors
Biosensors
bioinstrumentation
Peptide Receptors
field effect transistors
carbon nanotubes
Halogenated Diphenyl Ethers
retardants
chemistry
Poisons
congeners

Keywords

  • biosensor
  • environmental toxicant
  • Phage display
  • polybrominated diphenyl ether

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Selective and Sensitive Sensing of Flame Retardant Chemicals Through Phage Display Discovered Recognition Peptide. / Jin, Hyo Eon; Zueger, Chris; Chung, Woo Jae; Wong, Winnie; Lee, Byung Yang; Lee, Seung Wuk.

In: Nano Letters, Vol. 15, No. 11, 11.11.2015, p. 7697-7703.

Research output: Contribution to journalArticle

Jin, Hyo Eon ; Zueger, Chris ; Chung, Woo Jae ; Wong, Winnie ; Lee, Byung Yang ; Lee, Seung Wuk. / Selective and Sensitive Sensing of Flame Retardant Chemicals Through Phage Display Discovered Recognition Peptide. In: Nano Letters. 2015 ; Vol. 15, No. 11. pp. 7697-7703.
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