Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines

Young Ok Son, Kyung Yeol Lee, Jeong Chae Lee, Hyon Seok Jang, Jong Ghee Kim, Young Mi Jeon, Yong Suk Jang

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Considerable attention is being concentrated on dietary flavonoids in developing novel cancer-preventive approaches due to their potential ability to induce selective apoptosis of cancer cells. In this study, we prepared a flavonoid-containing fraction from a crude acetone extract of Rhus verniciflua Stokes (RVS), traditionally used as a food additive and as an herbal medicine, and named RVS chloroform-methanol fraction (RCMF). We evaluated the effects of RCMF on proliferation and apoptosis using mouse embryonic primary hepatic cells (MPHC), embryonic normal hepatic cell line (BNL CL.2), and its SV40-mediated transformed cell line (BNL SV A.8). We also investigated the effects of RCMF on the antioxidant defense system in those cells. This study demonstrated that RCMF exhibited a selective growth inhibition and apoptosis induction on transformed cells. BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of inducing growth inhibition and apoptosis of hepatic tumor cells.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalToxicology Letters
Volume155
Issue number1
DOIs
Publication statusPublished - 2005 Jan 15

Fingerprint

Rhus
Transformed Cell Line
Chloroform
Flavonoids
Methanol
Hepatocytes
Apoptosis
Cells
Food additives
Neoplasms
Food Additives
Herbal Medicine
Cytotoxicity
Growth
Acetone
Complex Mixtures
Glutathione
Tumors
Antioxidants
Cell Line

Keywords

  • Apoptosis
  • Flavonoids
  • Hepatocytes
  • Rhus verniciflua Stokes
  • Selective growth inhibition

ASJC Scopus subject areas

  • Toxicology

Cite this

Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. / Son, Young Ok; Lee, Kyung Yeol; Lee, Jeong Chae; Jang, Hyon Seok; Kim, Jong Ghee; Jeon, Young Mi; Jang, Yong Suk.

In: Toxicology Letters, Vol. 155, No. 1, 15.01.2005, p. 115-125.

Research output: Contribution to journalArticle

Son, Young Ok ; Lee, Kyung Yeol ; Lee, Jeong Chae ; Jang, Hyon Seok ; Kim, Jong Ghee ; Jeon, Young Mi ; Jang, Yong Suk. / Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. In: Toxicology Letters. 2005 ; Vol. 155, No. 1. pp. 115-125.
@article{26c76e7037d9432aa0f5ab70931f3774,
title = "Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines",
abstract = "Considerable attention is being concentrated on dietary flavonoids in developing novel cancer-preventive approaches due to their potential ability to induce selective apoptosis of cancer cells. In this study, we prepared a flavonoid-containing fraction from a crude acetone extract of Rhus verniciflua Stokes (RVS), traditionally used as a food additive and as an herbal medicine, and named RVS chloroform-methanol fraction (RCMF). We evaluated the effects of RCMF on proliferation and apoptosis using mouse embryonic primary hepatic cells (MPHC), embryonic normal hepatic cell line (BNL CL.2), and its SV40-mediated transformed cell line (BNL SV A.8). We also investigated the effects of RCMF on the antioxidant defense system in those cells. This study demonstrated that RCMF exhibited a selective growth inhibition and apoptosis induction on transformed cells. BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of inducing growth inhibition and apoptosis of hepatic tumor cells.",
keywords = "Apoptosis, Flavonoids, Hepatocytes, Rhus verniciflua Stokes, Selective growth inhibition",
author = "Son, {Young Ok} and Lee, {Kyung Yeol} and Lee, {Jeong Chae} and Jang, {Hyon Seok} and Kim, {Jong Ghee} and Jeon, {Young Mi} and Jang, {Yong Suk}",
year = "2005",
month = "1",
day = "15",
doi = "10.1016/j.toxlet.2004.09.003",
language = "English",
volume = "155",
pages = "115--125",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines

AU - Son, Young Ok

AU - Lee, Kyung Yeol

AU - Lee, Jeong Chae

AU - Jang, Hyon Seok

AU - Kim, Jong Ghee

AU - Jeon, Young Mi

AU - Jang, Yong Suk

PY - 2005/1/15

Y1 - 2005/1/15

N2 - Considerable attention is being concentrated on dietary flavonoids in developing novel cancer-preventive approaches due to their potential ability to induce selective apoptosis of cancer cells. In this study, we prepared a flavonoid-containing fraction from a crude acetone extract of Rhus verniciflua Stokes (RVS), traditionally used as a food additive and as an herbal medicine, and named RVS chloroform-methanol fraction (RCMF). We evaluated the effects of RCMF on proliferation and apoptosis using mouse embryonic primary hepatic cells (MPHC), embryonic normal hepatic cell line (BNL CL.2), and its SV40-mediated transformed cell line (BNL SV A.8). We also investigated the effects of RCMF on the antioxidant defense system in those cells. This study demonstrated that RCMF exhibited a selective growth inhibition and apoptosis induction on transformed cells. BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of inducing growth inhibition and apoptosis of hepatic tumor cells.

AB - Considerable attention is being concentrated on dietary flavonoids in developing novel cancer-preventive approaches due to their potential ability to induce selective apoptosis of cancer cells. In this study, we prepared a flavonoid-containing fraction from a crude acetone extract of Rhus verniciflua Stokes (RVS), traditionally used as a food additive and as an herbal medicine, and named RVS chloroform-methanol fraction (RCMF). We evaluated the effects of RCMF on proliferation and apoptosis using mouse embryonic primary hepatic cells (MPHC), embryonic normal hepatic cell line (BNL CL.2), and its SV40-mediated transformed cell line (BNL SV A.8). We also investigated the effects of RCMF on the antioxidant defense system in those cells. This study demonstrated that RCMF exhibited a selective growth inhibition and apoptosis induction on transformed cells. BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of inducing growth inhibition and apoptosis of hepatic tumor cells.

KW - Apoptosis

KW - Flavonoids

KW - Hepatocytes

KW - Rhus verniciflua Stokes

KW - Selective growth inhibition

UR - http://www.scopus.com/inward/record.url?scp=10044290769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10044290769&partnerID=8YFLogxK

U2 - 10.1016/j.toxlet.2004.09.003

DO - 10.1016/j.toxlet.2004.09.003

M3 - Article

VL - 155

SP - 115

EP - 125

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 1

ER -