Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines

Young Ok Son, Kyung Yeol Lee, Jeong Chae Lee, Hyon Seok Jang, Jong Ghee Kim, Young Mi Jeon, Yong Suk Jang

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Considerable attention is being concentrated on dietary flavonoids in developing novel cancer-preventive approaches due to their potential ability to induce selective apoptosis of cancer cells. In this study, we prepared a flavonoid-containing fraction from a crude acetone extract of Rhus verniciflua Stokes (RVS), traditionally used as a food additive and as an herbal medicine, and named RVS chloroform-methanol fraction (RCMF). We evaluated the effects of RCMF on proliferation and apoptosis using mouse embryonic primary hepatic cells (MPHC), embryonic normal hepatic cell line (BNL CL.2), and its SV40-mediated transformed cell line (BNL SV A.8). We also investigated the effects of RCMF on the antioxidant defense system in those cells. This study demonstrated that RCMF exhibited a selective growth inhibition and apoptosis induction on transformed cells. BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of inducing growth inhibition and apoptosis of hepatic tumor cells.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalToxicology Letters
Volume155
Issue number1
DOIs
Publication statusPublished - 2005 Jan 15

Keywords

  • Apoptosis
  • Flavonoids
  • Hepatocytes
  • Rhus verniciflua Stokes
  • Selective growth inhibition

ASJC Scopus subject areas

  • Toxicology

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