Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe3O4@SiO 3 nanoparticles: Application to comprehensive proteomic profiling

Arudra Palani, Je Sun Lee, Jiyoung Huh, Minsik Kim, Yeon Ji Lee, Jeong Ho Chang, Kwangyeol Lee, Sang Won Lee

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Superparamagnetic Fe3O4@SiO2 core-shell nanoparticles (ca. 30 nm diameter), which are surface- modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

Original languageEnglish
Pages (from-to)3591-3596
Number of pages6
JournalJournal of Proteome Research
Volume7
Issue number8
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Cysteine containing peptide enrichment
  • Proteomics
  • Superparamagnetic nanoparticle
  • Surface modification
  • Tandem mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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