Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe3O4@SiO 3 nanoparticles: Application to comprehensive proteomic profiling

Arudra Palani; Je Sun Lee; Jiyoung Huh; Minsik Kim; Yeon Ji Lee; Jeong Ho Chang; Kwangyeol Lee; Sang Won Lee

doi:10.1021/pr800067x

Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe₃O₄@SiO ₃ nanoparticles: Application to comprehensive proteomic profiling

Arudra Palani, Je Sun Lee, Jiyoung Huh, Minsik Kim, Yeon Ji Lee, Jeong Ho Chang, Kwangyeol Lee, Sang Won Lee

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Superparamagnetic Fe₃O₄@SiO₂ core-shell nanoparticles (ca. 30 nm diameter), which are surface- modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

Original language	English
Pages (from-to)	3591-3596
Number of pages	6
Journal	Journal of Proteome Research
Volume	7
Issue number	8
DOIs	https://doi.org/10.1021/pr800067x
Publication status	Published - 2008 Aug

Keywords

Cysteine containing peptide enrichment
Proteomics
Superparamagnetic nanoparticle
Surface modification
Tandem mass spectrometry

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

Access to Document

10.1021/pr800067x

Cite this

@article{0a1206edcd8149e0a1e16f889a54d918,

title = "Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe3O4@SiO 3 nanoparticles: Application to comprehensive proteomic profiling",

abstract = "Superparamagnetic Fe3O4@SiO2 core-shell nanoparticles (ca. 30 nm diameter), which are surface- modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.",

keywords = "Cysteine containing peptide enrichment, Proteomics, Superparamagnetic nanoparticle, Surface modification, Tandem mass spectrometry",

author = "Arudra Palani and Lee, {Je Sun} and Jiyoung Huh and Minsik Kim and Lee, {Yeon Ji} and Chang, {Jeong Ho} and Kwangyeol Lee and Lee, {Sang Won}",

year = "2008",

month = aug,

doi = "10.1021/pr800067x",

language = "English",

volume = "7",

pages = "3591--3596",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

number = "8",

}

TY - JOUR

T1 - Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe3O4@SiO 3 nanoparticles

T2 - Application to comprehensive proteomic profiling

AU - Palani, Arudra

AU - Lee, Je Sun

AU - Huh, Jiyoung

AU - Kim, Minsik

AU - Lee, Yeon Ji

AU - Chang, Jeong Ho

AU - Lee, Kwangyeol

AU - Lee, Sang Won

PY - 2008/8

Y1 - 2008/8

N2 - Superparamagnetic Fe3O4@SiO2 core-shell nanoparticles (ca. 30 nm diameter), which are surface- modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

AB - Superparamagnetic Fe3O4@SiO2 core-shell nanoparticles (ca. 30 nm diameter), which are surface- modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

KW - Cysteine containing peptide enrichment

KW - Proteomics

KW - Superparamagnetic nanoparticle

KW - Surface modification

KW - Tandem mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=53049110314&partnerID=8YFLogxK

U2 - 10.1021/pr800067x

DO - 10.1021/pr800067x

M3 - Article

C2 - 18563925

AN - SCOPUS:53049110314

SN - 1535-3893

VL - 7

SP - 3591

EP - 3596

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 8

ER -