Selective inhibition of collagen synthesis by okadaic acid in cultured human fibroblasts

In-San Kim, Rang Woon Park, Kun Young Sohn, Joon Seung Jo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To determine whether protein phosphatases can affect collagen synthesis, we examined the effect of okadaic acid, a potent specific inhibitor of protein phosphatases 1 and 2A, on collagen synthesis. Okadaic acid significantly decreased the [3H]proline incorporation into the collagenase-digestible protein and the percent collagen synthesis. These effects were synergistic with phorbol myristate acetate (PMA). The time course study showed that okadaic acid inhibited collagen synthesis after a 12 h treatment while PMA inhibited at 3 h. Down-regulation of protein kinase C by chronic treatment with PMA did not abrogate the okadaic acid-dependent inhibition. These results provide evidence for the involvement of protein phosphatases in the regulation of collagen synthesis.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalBiochemical and biophysical research communications
Volume199
Issue number1
DOIs
Publication statusPublished - 1994 Feb 28
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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